Best practices in designing, sequencing, and identifying random DNA barcodes
… We conclude this section with a brief discussion of “pre-multiplexing,” a way of leveraging …
is that any sufficiently large population harbors beneficial genetic variation, even prior to the …
is that any sufficiently large population harbors beneficial genetic variation, even prior to the …
[PDF][PDF] Structural variant detection in region-based phased diploid assemblies from linked-reads
C Luo, X Zhou - The International symposium on bioinformatics …, 2021 - lab.vanderbilt.edu
… and other applications through highly-multiplexed genome … Structural variants (SVs)
represent large genomic variations … process since it divides barcoded reads into two different …
represent large genomic variations … process since it divides barcoded reads into two different …
The continuing evolution of barcode applications: Functional toxicology to cell lineage
M Fasullo, M Dolan - Experimental Biology and Medicine, 2022 - journals.sagepub.com
… using signature-tagged mutagenesis (STM). Pertinent to the focus of this review is the use of
barcoding to identify … Multiplexing allows for multiple conditions to be tested individually and …
barcoding to identify … Multiplexing allows for multiple conditions to be tested individually and …
Sequencing barcode construction and identification methods based on block error-correction codes
… resistant to multiple types of errors in multiplexed sequencing, we propose a novel barcode
… sequence. Furthermore, we propose a soft decision identification method for sequencing …
… sequence. Furthermore, we propose a soft decision identification method for sequencing …
[HTML][HTML] Ultrasensitive sequencing of STR markers utilizing unique molecular identifiers and the SiMSen-Seq method
M Sidstedt, AH Gynnå, KM Kiesler, L Jansson… - … International: Genetics, 2024 - Elsevier
… -Seq (Simple, multiplexed, PCR-based barcoding of DNA for sensitive mutation detection
using sequencing) … The seven STR multiplex assay developed in this study is based on the …
using sequencing) … The seven STR multiplex assay developed in this study is based on the …
[PDF][PDF] Limitations and challenges of genetic barcode quantification
… using the Multiplex PCR Plus Kit (Qiagen), according to manufacturer’s protocol, with 57 C
as annealing temperature, 0.2 pmol of P5-Dual-index primer/Multiplex-… four barcoded cell …
as annealing temperature, 0.2 pmol of P5-Dual-index primer/Multiplex-… four barcoded cell …
Genotyping by sequencing advancements in barley
NR Rajendran, N Qureshi… - Frontiers in plant …, 2022 - frontiersin.org
… Genetic variations in the traits of interest in crops are vital for their improvement. DNA markers
have been widely used to estimate these variations in … Multiplex sequencing by adding …
have been widely used to estimate these variations in … Multiplex sequencing by adding …
[PDF][PDF] BaM-seq and TBaM-seq, highly multiplexed and targeted RNA-seq protocols for rapid, low-cost library generation from bacterial samples
GE Johnson, DJ Parker, JB Lalanne… - NAR Genomics and …, 2023 - academic.oup.com
… bacterial-multiplexed-seq (TBaM-seq) that allows for differential expression analysis of
specific gene panels with … (2008) Identification of genetic variants using bar-coded multiplexed …
specific gene panels with … (2008) Identification of genetic variants using bar-coded multiplexed …
Synthetic DNA barcodes identify singlets in scRNA-seq datasets and evaluate doublet algorithms
… can identify ground-truth singlets and enable robust doublet detection in non-barcoded …
We first replicated findings from the benchmarking study4,42 on the same non-barcoded …
We first replicated findings from the benchmarking study4,42 on the same non-barcoded …
A MinION-based Long-Read Sequencing Application With One-Step PCR for the Genetic Diagnosis of 21-Hydroxylase Deficiency
E Adachi, R Nakagawa… - The Journal of …, 2024 - academic.oup.com
… technology for genetic analysis of CYP21A2 is the multiplex ligation… detection of large and
complex genomic variants and … To achieve cost efficiency, we barcoded the PCR products and …
complex genomic variants and … To achieve cost efficiency, we barcoded the PCR products and …