The aim of the present work is to evaluate the ability of ‘fungi’ for the biodegradation of recalcitrant xenobiotic compound, ‘Atrazine’ in batch liquid cultures. Different parameters like pH (2.0–8.0) temperature (16–32 °C), biomass (1–5 g), and concentration (25–100 ppm) were optimized for the efficient degradation of atrazine. The decomposition behavior of atrazine is analyzed with the help of Fourier Transform Infrared (FTIR) spectroscopy. Herein, we have reported that the Bjerkandera adusta possess high removal efficiency of the xenobiotic compound (atrazine) up to 92%. The fungal strain investigated could prove to be a valuable active pesticide degrading micro-organism, with high detoxification values. These results are useful for improved understanding and prediction of the behavior and fate of B. adusta in the bio-purification of wastewater contaminated with xenobiotics. Thus providing a new and green approach for the remediation of toxicants without altering the environmental components.