… biological systems and prediction what effect will a specific Biobrick have in a new system. …
the existing ones, synthetic biologists are combining these Biobricks as if building a wall or a …

… For example, new state spaces for exploration can be provided simply by modifying and
re-engineering of the environment. Other mechanisms could be exploitation of exaptation, …

… Bio-Brick’ assembly have been the first method to assemble biological standard part, it carries
its own limitations [22, 23]. Apart from Bio-Brick … re-wiring and re-engineering of metabolic …

… the BioBrick system lies in the assembly standards that govern the construction of BioBrick
… In-fusion BioBrick assembly and re-engineering. Nucleic Acids Research, 38(8), 2624–2636. …

… However, alternative multipartite assembly methods have been developed based on the
BioBrick/… /FungalBraid assemblies [181,182,183,184]. In these cases, genetic constructs can be …

Transgenesis, which inserts exogenous DNA into animal genomes, is a widely used technique.
Traditionally, the constructs for transgenesis are generated by step-by-step subcloning of …

… used in BioBrick TM assembly and modern one-step DNA assembly techniques 1–7 ; (II)
viral vectors for gene delivery 8,9 ; (III) virus-induced gene silencing (VIGS) 10,11 ; (IV) the RNA …

… In-Fusion cloning uses a proprietary version of vaccinia virus-encoded DNA polymerase
called the In-Fusion … In the In-Fusion system, the regions of homology are generated by adding …

… BioBrick Assembly Standard. In this system, parts (eg, promoters, RBSs, CDSs, and terminators)
called BioBricks … In the original BioBrick standard, when two genetic parts are assembled…

… In this study, we developed an In-fusion based gene stacking strategy for transgene stacking
and a multi-gene vector harbouring 6 mogrosides biosynthesis genes and transformed it …