Recombineering: Genetic Engineering in Escherichia coli Using Homologous Recombination
LC Thomason, N Costantino, X Li, DL Court - Current Protocols, 2023 - Wiley Online Library
… increases the recovery of RecET plasmid recombinants, … class by plasmid DNA isolation and
re-transformation at a DNA … has been used to isolate recA mutations. For such screening to …
re-transformation at a DNA … has been used to isolate recA mutations. For such screening to …
RecA‐independent recombination: Dependence on the Escherichia coli RarA protein
K Jain, EA Wood, ZJ Romero… - Molecular microbiology, 2021 - Wiley Online Library
… Whereas most detectable homologous recombination events … (b) Plasmids were isolated
from Tet R recombinants from the … Increases in RecA and RarA-independent recombination …
from Tet R recombinants from the … Increases in RecA and RarA-independent recombination …
RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation
M Skutel, D Yanovskaya, A Demkina… - Nucleic Acids …, 2024 - academic.oup.com
… in plasmid DNA via homologous recombination generates … of RecA and RecBCD proteins
using appropriate mutants. The … substrates and promotes Chi-independent recombination (66,…
using appropriate mutants. The … substrates and promotes Chi-independent recombination (66,…
[PDF][PDF] DNA ADP-ribosylation stalls replication and is reversed by RecF-mediated homologous recombination and nucleotide excision repair
… replication and leads to an increase in RecA levels, indicating … Instead, we isolated a mutated
version of DarT containing a … that NER activity is independent of RecF following UV light …
version of DarT containing a … that NER activity is independent of RecF following UV light …
Molecular and functional characterization of the Listeria monocytogenes RecA protein: insights into the homologous recombination process
… plasmid DNA purification kit, gel extraction kit, RNA isolation kit… recA was introduced by site
directed mutagenesis using the kit … of LmRecA also increases in response to DNA damage, L. …
directed mutagenesis using the kit … of LmRecA also increases in response to DNA damage, L. …
DNA damage-signaling, homologous recombination and genetic mutation induced by 5-azacytidine and DNA-protein crosslinks in Escherichia coli
JA Klaric, DJ Glass, EL Perr, AD Reuven… - … of Mutagenesis, 2021 - Elsevier
… coli CMeT, Dcm; however, we also find evidence for Dcm-independent DNA … increase in
mutation frequency by Pol IV when induced. Induction of Pol II caused about a 3-fold increase in …
mutation frequency by Pol IV when induced. Induction of Pol II caused about a 3-fold increase in …
Mechanism of RecF–RecO–RecR cooperation in bacterial homologous recombination
S Nirwal, M Czarnocki-Cieciura, A Chaudhary… - Nature Structural & …, 2023 - nature.com
… the DNA binding: RecF–DNA interface mutants (d), RecR-K23A … to the boundary of the DNA
gap enhances its function. … Bayesian polishing (with re-extraction with an unbinned pixel size …
gap enhances its function. … Bayesian polishing (with re-extraction with an unbinned pixel size …
[HTML][HTML] Natural transformation in Deinococcus radiodurans: a genetic analysis reveals the major roles of DprA, DdrB, RecA, RecF, and RecO proteins
S Ithurbide, G Coste, J Lisboa, N Eugénie… - Frontiers in …, 2020 - frontiersin.org
… (IIIb) In a RecA independent manner, DdrB, via its single … of the incoming DNA by homologous
recombination, we observed … by the mutant counterpart via homologous recombination. D. …
recombination, we observed … by the mutant counterpart via homologous recombination. D. …
Recombination proteins differently control the acquisition of homeologous DNA during Bacillus subtilis natural chromosomal transformation
E Serrano, C Ramos, JC Alonso… - Environmental …, 2021 - Wiley Online Library
… occurs via RecA-dependent homologous recombination (HR) … of donor DNA with increased
SD in different rec mutants. The … RecA, in concert with SsbA, DprA and RecX, promotes DNA …
SD in different rec mutants. The … RecA, in concert with SsbA, DprA and RecX, promotes DNA …
Homologous recombination repair intermediates promote efficient de novo telomere addition at DNA double-strand breaks
A Davé, CC Pai, SC Durley, L Hulme… - Nucleic Acids …, 2020 - academic.oup.com
… rad55Δ double mutant resulted in a striking increase in LOH (… the MATa site from five
individually isolated ade+ G418S LOH … Our study identifies an independent role for the HR …
individually isolated ade+ G418S LOH … Our study identifies an independent role for the HR …