… of treatment options to reduce their anemia and transfusion dependence… Fetal hemoglobin
(HbF) is a hemoglobin synthesized during fetal life that functions similarly to adult hemoglobin …

… the hemoglobin tetramer and deformation of RBCs. This ‘sickling’ of RBCs leads to hemolytic
anemia… Control of human hemoglobin switching by LIN28B-mediated regulation of BCL11A …

… shRNA-mediated IMP1 knockdown in the fetal-like HUDEP-1 … Hereditary persistence of
fetal haemoglobin HR … In adult humans, RBCs are produced from pluripotent haematopoietic …

… for carrying oxygen in adult blood, consists of … During fetal development, however, erythrocytes
preferentially express an alternative hemoglobin tetramer called fetal hemoglobin (HbF; …

… We also observed lower red blood cell (RBC) counts and … relative to adults with an overall
lower hemoglobin content (… ectopic expression in mature adult HSCs can induce fetal-like …

… Finally, we then explored how a LIN28Bmediated increase in … In order to investigate its
importance, we turned to ex vivo … and production of fetal-like erythrocytes from adult human …

… ineffective erythropoiesis, defective red blood cells (RBC) … that miRNAs can elevate fetal
haemoglobin (HbF) levels via … In human ex vivo erythropoiesis, overexpression of miRNA-…

… reticulocytes have the same dry mass content as RBCs, ie, 0.35 g dry mass of hemoglobin
per ml of cell, and using the measured volume of 72 G 12 mm3 (Figure 3B), we find that each …

… the state of mature RBCs. Continuous … vivo erythropoiesis taking place in erythropoietic
islands of bone marrow41, as well as improve our ability to manufacture red blood cells in ex vivo …

… genetically modified to produce non-pathogenic RBCs by lentivi… -level HbF induction possibly
decreasing erythropoiesis from … in ex vivo gene-addition/editing therapy as well as in vivo …