… 89 Zr is ideal for radiolabeling full antibodies (IgGs) because … A separate cohort of three
mice was harvested at 1 h post … Surface-bound and internalized fractions were expressed as …

… (immunoPET) can identify targets for cell-signaling independent … Cell surface-bound radiotracer
was collected by cells … Internalized fraction was obtained after cell lysis with 1 M sodium …

… and biodistribution challenges, which limit their overall clinical … not distinguish between
normal healthy proliferating cells … at least one internal aqueous compartment surrounded by one …

… Therefore, these surface-bound microparticles promote … which make up the internal
structure of the cell, are essential for … A minimum particle size difference of three times is often …

… buffer (0.1 M, pH 5) was added to the radiolabeling mixture until a total … The activity in both
cell surface-bound and internalized … difference between the internalization, surface bound and …

… the surface-bound agents, the cells were … in separate Eppendorf tubes and measured on
a gamma counter to determine the percentage of unbound, membrane bound, and internalized …

… removed by washing cells and surface bound NCs were … and we would observe no difference
between NCs targeted to … transferrin was simultaneously internalized into the cells, thus …

… to distinguish between surface bound and internalised materials and are … limits the use of
phenotyping antibodies in mixed cell … enzyme and followed by a gel extraction and purification …

… , this cell line was used in this work to assess the differences … CS NPs with a surface bound
targeting ligand that can easily … NPs were specifically internalized by cells expressing TfR via …

… the cell surface bound fraction was 7 times lower (15.6 %IA per 10 6 C4-2 cells) and internalized
fraction was 4 times lower (… However, in the present study, no differences were detected …