[HTML][HTML] Fluorescence Lifetime Imaging Microscopy (FLIM) reveals spatial-metabolic changes in 3D breast cancer spheroids

K Karrobi, A Tank, MA Fuzail, M Kalidoss, K Tilbury… - Scientific Reports, 2023 - nature.com
multiphoton FLIM to investigate the changes in cellular metabolism of 3D breast spheroids
derived from MCF-10A … a malignant phenotype in MCF-10A cells through YAP/TAZ activation …

Metabolic characterization of breast cancer cells and extracellular vesicles using fluorescence lifetime imaging microscopy

EM Martin, JE Sorrells, E Aksamitiene, P Mukherjee… - Cancer Research, 2022 - AACR
Two-photon fluorescence lifetime imaging microscopy (FLIM), a label-free metabolic imaging
technique that can spatially map the … human breast cells (MCF10A) and breast cancer cells (…

Fast fluorescence lifetime imaging microscopy using single-and multi-photon peak event detection for rapid quantification of NAD (P) H-related metabolic dynamics …

JE Sorrells, M Marjanovic, RR Iyer… - Multiphoton …, 2023 - spiedigitallibrary.org
multiphoton autofluorescence lifetime of NAD(P)H is a convenient contrast for quantitatively
mapping cellular metabolism … and MCF10A cells, the mean fluorescence lifetime increases to …

Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity

EA Shirshin, MV Shirmanova… - Proceedings of the …, 2022 - National Acad Sciences
… Here, we demonstrate that fluorescence lifetime imaging with … assessment of cellular metabolic
heterogeneity in living cells. … Mapping and quantification of the metabolic heterogeneity at …

Multimodal Phasor Approach to study breast cancer cells invasion in 3D spheroid model

G Tedeschi, F Palomba, L Scipioni, MA Digman - bioRxiv, 2024 - biorxiv.org
cancer and non-tumorigenic (MCF-10A) cells and a distinct … Fluorescence lifetime images
were collected with a Zeiss … laser (Spectra-Physics) for two-photon excitation, tuned at 720 nm (…

Exploring tumor metabolism with time-resolved fluorescence methods: from single cells to a whole tumor

MV Shirmanova, VI Shcheslavskiy, MM Lukina… - … diagnostics of cancer, 2020 - Springer
Fluorescence lifetime imaging on a microscopic scale … the metabolic activity of the same
cervical cancer cells using … for two-photon probing of xenograft tumors in mice using MCA 207 …

Autofluorescence imaging to evaluate cellular metabolism

A Theodossiou, L Hu, N Wang, U Nguyen… - JoVE (Journal of …, 2021 - jove.com
… the lifetime image by integrating the fluorescence lifetime decay. … Multiphoton fluorescence
lifetime imaging of NAD(P)H and FAD allows visualization of cell morphology and metabolism

[HTML][HTML] Evaluation of cell metabolic adaptation in wound and tumour by Fluorescence Lifetime Imaging Microscopy

D Morone, FD Autilia, T Schorn, M Erreni, A Doni - Scientific reports, 2020 - nature.com
… Measurement of NADH fluorescence lifetime was therefore extended in an experimental
transplantable model of cancer in order to reconstruct a cellular metabolic map of macrophages …

Label-free metabolic imaging in vivo by two-photon fluorescence lifetime endomicroscopy

W Liang, D Chen, H Guan, HC Park, K Li, A Li… - ACS …, 2022 - ACS Publications
… potential of NADH fluorescence lifetime imaging microscopy (… developed two-photon
fluorescence (2PF) lifetime imaging … kidney from the surrounding fat tissue using cotton swabs, the …

[HTML][HTML] Label-free spatially maintained measurements of metabolic phenotypes in cells

L Hu, N Wang, JD Bryant, L Liu, L Xie… - Frontiers in …, 2023 - frontiersin.org
Fluorescence lifetime images of two endogenous metabolic … acquired by a multi-photon
fluorescence lifetime microscope and … pathway perturbations of cancer cells using both chemical …