A metabolic labeling method detects m6A transcriptome-wide at single base resolution
X Shu, J Cao, M Cheng, S Xiang, M Gao, T Li… - Nature chemical …, 2020 - nature.com
… immunoprecipitation (IP)-based sequencing method (named m 6 … cellular metabolic a 6
A-labeled RNAs. During our search, we … , we speculated that m 6 A mRNA targets with a fast …
A-labeled RNAs. During our search, we … , we speculated that m 6 A mRNA targets with a fast …
[HTML][HTML] Analysis of RNA modifications by second-and third-generation deep sequencing: 2020 update
Y Motorin, V Marchand - Genes, 2021 - mdpi.com
… In addition to classical RNA techniques, such as 5’/3’ and specific internal labeling as well …
is rapidly developing and improving in reliability, but it has still not reached maturity for routine …
is rapidly developing and improving in reliability, but it has still not reached maturity for routine …
Mapping the epigenetic modifications of DNA and RNA
… rapidly evolving field. We focus on high-throughput detection … -based and chemical labeling
sequencing methods have been … a basic site at m 7 G positions, which can be further labeled …
sequencing methods have been … a basic site at m 7 G positions, which can be further labeled …
Detecting DNA and RNA and differentiating single-nucleotide variations via field-effect transistors
… RNA sequences and to distinguish from RNA sequences with single nucleotide variations.
The development and implementation of electronic biosensors that rapidly … labeling and/or …
The development and implementation of electronic biosensors that rapidly … labeling and/or …
[HTML][HTML] Recent advances in the detection of base modifications using the Nanopore sequencer
L Xu, M Seki - Journal of human genetics, 2020 - nature.com
… DNA and RNA modifications have important functions, … Existing methods based on short-read
sequencing for the detection of … Nascent RNAs are labeled by pulse labeling using base …
sequencing for the detection of … Nascent RNAs are labeled by pulse labeling using base …
[HTML][HTML] Covalent labeling of nucleic acids
N Klöcker, FP Weissenboeck… - Chemical Society Reviews, 2020 - pubs.rsc.org
… Depending on the approach, site-, sequence- and cell-specificity can be achieved. DNA and
RNA labeling are rapidly developing fields that bring together multiple areas of research: on …
RNA labeling are rapidly developing fields that bring together multiple areas of research: on …
Identification of differential RNA modifications from nanopore direct RNA sequencing with xPore
PN Pratanwanich, F Yao, Y Chen, CWQ Koh… - Nature …, 2021 - nature.com
… Here we develop a computational method, xPore, to identify differential RNA modifications
from nanopore direct RNA sequencing (RNA-seq) data. We evaluate our method on …
from nanopore direct RNA sequencing (RNA-seq) data. We evaluate our method on …
[HTML][HTML] Fast microwave heating-based one-step synthesis of DNA and RNA modified gold nanoparticles
… To more detailly evaluate the role of T base in labeling process, we replaced one or two T
bases with A, C, or G base in poly (T 5 ) -tagged DNA sequences. Results showed that in most …
bases with A, C, or G base in poly (T 5 ) -tagged DNA sequences. Results showed that in most …
HydraPsiSeq: a method for systematic and quantitative mapping of pseudouridines in RNA
V Marchand, F Pichot, P Neybecker… - Nucleic acids …, 2020 - academic.oup.com
… sequencing-based methods for RNA modification mapping combine both single-nucleotide
resolution and precise quantification, since multiple … at positions (labelled in green and blue) …
resolution and precise quantification, since multiple … at positions (labelled in green and blue) …
[HTML][HTML] Detection of m6A from direct RNA sequencing using a multiple instance learning framework
… RNA molecule is missing. Here we present m6Anet, a neural-network-based method that
leverages the multiple … handle missing read-level modification labels in site-level training data. …
leverages the multiple … handle missing read-level modification labels in site-level training data. …