Whole‐Genome Regulation for Yeast Metabolic Engineering

S Jiang, T Si, J Dai - Small Methods, 2020 - Wiley Online Library
… This work demonstrates the capacity of the automated multiplex genome-scale engineering
… for multiplexed accurate genome editing with short, trackable, integrated cellular barcodes (…

[HTML][HTML] High-throughput analysis of adaptation using barcoded strains of Saccharomyces cerevisiae

VJ Fasanello, P Liu, CA Botero, JC Fay - PeerJ, 2020 - peerj.com
… utilized a pool of half a million barcoded yeast strains to detect and track the fate of lineages
and to … We highlight the advantages of multiplexing samples with indexing as well as the …

Integration of DNA barcoding and nanotechnology in drug delivery

M Kantak, P Batra, P Shende - International Journal of Biological …, 2023 - Elsevier
… novel tool for identification and multiplex structural detection of … ), bacterial 16S, and
fungal-transcribed spacer barcoded loci. 1-… continuous progress in gene editing technology for the …

Genetic barcodes allow traceability of CRISPR/Cas9-derived Aspergillus niger strains without affecting their fitness

S Garrigues, RS Kun, RP de Vries - Current Genetics, 2021 - Springer
… of the fastest-growing technologies for genome editing in a range of … CRISPR/Cas9 allows
for precise edits to DNA in living cells, … Genomic DNA was obtained from fungal mycelia using …

Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology

Y Zeng, Y Hong, F Azi, Y Liu, Y Chen, C Guo… - Current Opinion in …, 2022 - Elsevier
Trackable multiplex recombineering (TRMR) with barcoded DNA library can be used to rapidly
screen genomic … and multiplexed genome engineering technology in yeast, and enabled …

Optimizing Nucleic Acid Delivery Systems through Barcode Technology

S Park, M Kim, JW Lee - ACS Synthetic Biology, 2024 - ACS Publications
… (32) A universal primer sequence that does not bind to mouse or human genomic DNA was
used for QUANT barcode design. Universal primer sites were created to reduce secondary …

[图书][B] Mapping of Causal Variants Through Precision Genome Editing

SAA Chen - 2022 - search.proquest.com
yeast genome editing system that can make precise single … Illumina sequencing adaptors
with custom barcode indices (P5… and throughput via multiplexed precision genome editing. …

Optical DNA Mapping of the Saccharomyces cerevisiae Genome

H Zachrisson - 2023 - odr.chalmers.se
… de novo optical genome assembly of the yeast Saccharomyces … yeast cells was performed
using the CHEF Yeast Genomicbarcodes generated from the data collected by the multiplex

Barcoded bulk QTL mapping reveals highly polygenic and epistatic architecture of complex traits in yeast

ANN Ba, KR Lawrence, A Rego-Costa… - Elife, 2022 - elifesciences.org
barcoding of every strain, which allows us to conduct sequencing- based bulk phenotype
measurements; (ii) a highly multiplexed … We find that the genotyping is accurate, with detectable …

DNA Barcoding Technology for Lineage Recording and Tracing to Resolve Cell Fate Determination

IS Kim - Cells, 2023 - mdpi.com
… single-cell barcoding technologies with genomic sequencing … Liu and colleagues searched
for a new base editor in yeast … of the barcode becomes a decisive factor in ensuring accurate