… As a complementary approach, reactivity-based chemical … lysine, which can reveal ‘ligandable’
hot spots in proteins with … al [53] showed that NHS esters primarily target lysine residues …

… proteome by using reactivity-based chemical probes and advanced … probes that react
with amino acid hotspots within proteins to map proteome-wide reactive and ligandable …

… being deployed on a proteome-wide scale thereby expanding … 4B) as versatile ABPP probes
to profile ligandable protein … to estimate warhead reactivity based on quantum mechanics (…

… probes to monitor a total of nine different amino acids as well as the N-terminus proteome-wide. …
Global profiling of lysine reactivity and ligandability in the human proteome. Nat. Chem. …

… , we show that NHS-esters are viable chemoproteomic probes, … ) as a versatile reactivity-based
probe for mapping the reactivity … for mapping the reactivity of proteome-wide accessible …

… by chemical probes is used for proteome-wide profiling of … As such, the reactivity-based
probe with only the NHS ester … This is consistent with prior observations that NHS esters can be …

… , ABPs/reactivity-based probes and photoaffinity probes will … approaches to map proteome-wide
reactive cysteine and lysine … hotspots within proteins to explore potential ligandable sites …

… proteome-wide by leveraging reactive chemical probes and … the ligandable lysine proteome,
and thus constitute versatile ‘… We also show here how integrating these ligandability maps …

… NHS esters are widely used reactive species for efficiently and specifically coupling primary
… A similar alkyne-functionalized NHS ester has been used as a versatile activity-based probe …

… In addition, we demonstrate the versatility of this reagent to … range of nucleophilic ligandable
hotspots, including tyrosine, … thiol reactivity, and map proteome-wide lysine hotspots. The …