Formalin fixation and paraffin embedding interfere with the preservation of optical metabolic assessments based on endogenous NAD (P) H and FAD two-photon …

A Sánchez-Hernández, CM Polleys… - Biomedical Optics …, 2023 - opg.optica.org
… of NADH and NADPH are very similar, we use NAD(P)H to … However, in vivo imaging is
challenging and excised tissues … Nakase, “Oxidation-reduction ratio studies of mitochondria in …

[HTML][HTML] Investigating the ischaemic PHASE of skin NADH fluorescence dynamics in recently diagnosed primary hypertension: A time series analysis

R Pawlak-Chomicka, W Chomicki, T Krauze… - Journal of Clinical …, 2023 - mdpi.com
… Notably, this dysfunction is observable in an in vivo non-invasive clinical model with … of
NADH during skin ischaemia can be non-invasively studied by the FMSF method. The clinical

Purification and characterization of NADP-isocitrate dehydrogenase from skeletal muscle of Urocitellus richardsonii

IA MacLean, A Varma, KB Storey - Molecular and Cellular Biochemistry, 2023 - Springer
… , maintenance of proper oxidationreduction balance, and … All protocols described above
for animal holding and … the rate of production of NADH by measuring absorbance at 340 …

[HTML][HTML] Nicotinamide riboside—the current state of research and therapeutic uses

M Mehmel, N Jovanović, U Spitz - Nutrients, 2020 - mdpi.com
… importance in cellular oxidationreduction reactions, including … the myocardial NAD + /NADH
ratio and exhibits protective … and SIRT3 activities increased in vitro and in vivo [37], which …

Developing optical imaging tools to investigate metabolic and structural biomarkers in rodent injury models

S Mostaghimi - 2021 - search.proquest.com
… We also define the redox ratio (NADH/FAD) as a biomarker to … It showed that our in vivo
fluorescence imager could … -animal models[27]. As a quantitative biomarker, the ratio of …

[PDF][PDF] Evolving concepts in NAD+ metabolism

CCS Chini, JD Zeidler, S Kashyap, G Warner, EN Chini - Cell metabolism, 2021 - cell.com
… in a myriad of oxidation-reduction reactions including the electron … -blocking antibody in vivo
and in vitro. Our results demonstrate … that increasing the NAD+/NADH ratio in RS by ectopic …

Exploring tumor metabolism with time-resolved fluorescence methods: from single cells to a whole tumor

MV Shirmanova, VI Shcheslavskiy, MM Lukina… - … optical diagnostics of …, 2020 - Springer
oxidation-reduction states of mitochondria taking the ratio of the fluorescence intensities of
oxidized flavoproteins to reduced NADH … metabolism with neoplasia development in vivo [43]. …

[HTML][HTML] Understanding cellular redox homeostasis: A challenge for precision medicine

V Tretter, B Hochreiter, ML Zach, K Krenn… - International journal of …, 2021 - mdpi.com
… amino acid oxidation generate the reducing equivalents NADH and FADH 2 , which are the
… success in vitro and in animal models. However, as always, the next steps to clinical trials in …

[PDF][PDF] The 80th Anniversary of Cortical Spreading Depression of Leão: A Major Component in Experimental and Clinical Neuropathology

A Mayevsky - British Journal of Healthcare and Medical …, 2023 - scholar.archive.org
… fluorescence in anaesthetized or unanaesthetized animals, a … the metabolic states of the
mitochondria evaluated in vitro (… ' monitoring, we want to use the NADH trace as an indicator to …

[HTML][HTML] Non-invasive quantification of the mitochondrial redox state in livers during machine perfusion

RJ de Vries, SEJ Cronin, P Romfh, CA Pendexter… - Plos one, 2021 - journals.plos.org
… metabolically active whole organs ex vivo that we leveraged in … The animals were maintained
in accordance with National … we measured the NAD, NADH, NADP, NADPH concentrations …