… design and site-directed mutagenesis transformed human serum recombinant
butyrylcholinesterase (BChE) into a highly efficient cocaine … a plant-produced cocaine-hydrolase …

… cocaine, and its variants with improved cocaine hydrolase (… hydrolysis rate have also
been rational designed to reduce the … The rBChE expressed in plants and goat milk was also …

… study acetylcholinesterase and butyrylcholinesterase structure and … Rate-determining steps
can be made faster with residue … intensity and increasing binding activity of thioflavin-T with …

… is unable to pass through pores of the plant cell wall (35–50 nm) [… by the fusion of cocaine
hydrolase (a huBChE mutant … proteins designed as native analogues, including improved half-…

… that can effectively modulate activity and stability. … Cocaine Hydrolase Variants of
Butyrylcholinesterase Used in This … on an enzyme system plant-derived cocaine hydrolase …

… 6 and obidoxime are mainly intended for the reactivation of AChE … The PROSS system
proposed 7 variants of BChE that were … In cases of enzymes from silkworms and transgene plants, …

… variant forms of the enzyme with little or no hydrolytic activity … of enzymes, is a part of the
serine hydrolase superfamily[1,2]. … therapy are being developed against cocaine addiction, and …

… mutagenesis was used to design NCS variants with the aim … that the catalytic efficiency of
butyrylcholinesterase (BchE, an … plant-derived natural products are currently being developed…

… recombinant enzyme was designed and produced using … For instance, plant-derived BChE,
created problems due to … to function with elevated hydrolase activity and also slowing down …

… Specifically, they determined the biological activity of plant-… stories include long-acting
cocaine hydrolases (CocHs) that … a rationally designed mutant of IgG-1 Fc has improved the …