Chromatographic tools for plant-derived recombinant antibodies purification and characterization

C Temporini, R Colombo, E Calleri, S Tengattini… - … of pharmaceutical and …, 2020 - Elsevier
… platforms for recombinant protein production as alternative to bacteria, yeast or mammalian
systems. … ) and on the degree of protein degradation (proteolysis). As for mAbs produced in …

Improving transient protein expression in agroinfiltrated Nicotiana benthamiana

K Beritza, EC Watts, RAL van der Hoorn - New Phytologist, 2024 - Wiley Online Library
… increased yields for various recombinant proteins achieved with … systems, such as
mammalian cell culture and microbial … with Alum elicits protective immune responses in mice. …

[HTML][HTML] Optimising expression and extraction of recombinant proteins in plants

RJ Coates, MT Young, S Scofield - Frontiers in Plant Science, 2022 - frontiersin.org
… protein production, and while bacterial and mammalian cell … and antibodies, which are
produced as recombinant proteins … , which can be done by protecting the protein, inhibiting …

Innovation in plant-based transient protein expression for infectious disease prevention and preparedness

F Sainsbury - Current opinion in biotechnology, 2020 - Elsevier
… This strategy can result in a 1.4-fold increase in recombinant antibody … for the protection
of recombinant proteins [34 • ]. RNAi constructs have also been used to knock down proteolytic

[HTML][HTML] Co-expression with replicating vector overcoming competitive effects derived by a companion protease inhibitor in plants

J Ma, X Ding, Z Li, S Wang - Frontiers in Plant Science, 2021 - frontiersin.org
… strategy to prevent RP from proteolytic degradation in plants. … or obfuscate the in situ protective
effects of a companion PI. … tPA antibody was used to immunodetect the recombinant rPA …

Optimizing product quality in molecular farming

A Schiermeyer - Current Opinion in Biotechnology, 2020 - Elsevier
proteolytic processing of recombinant target molecules and strategies have been devised to
protect … properties to Fcγ receptors compared to VRC01 derived from mammalian HEK cells. …

Characterization of Nicotiana benthamiana subtilisin-like peptidases

A Vrkić - 2021 - repozitorij.unizg.hr
… Even though bacterial and mammalian cells are still the most … P69 SBTs are involved in
pathogen defence as modulators … of recombinant glycoproteins such as monoclonal antibodies. …

Transient expression of recombinant proteins in plants using potato virus X based vectors

ES Mardanova, NV Ravin - Methods in enzymology, 2021 - Elsevier
… identical to those occurring in mammalian cells. Recombinant proteins can be produced in
… This method allows efficient producing of recombinant proteins within days-weeks timescale…

[PDF][PDF] … of the IntF2A fusion domain-based bicistronic vector for simultaneous expression of the polypeptide chains of bevacizumab antibody and investigating its …

P Andaz, M Jafari - Agricultural Biotechnology Journal, 2024 - academia.edu
… by recombinant DNA technology in a mammalian expression … benefits for producing
recombinant pharmaceutical proteins. … consideration in designing antibody expression vectors. …

[HTML][HTML] A rationally designed bovine IgA Fc scaffold enhances in planta accumulation of a VHH-Fc fusion without compromising binding to enterohemorrhagic E. coli

A Chin-Fatt, R Saberianfar, R Menassa - Frontiers in Plant Science, 2021 - frontiersin.org
… The negative impact of endogenous proteases on the yields of plant recombinant proteins
… needed for protection as the secretory form is the predominant functional unit in mammals (…