A coarse-grained NADH redox model enables inference of subcellular metabolic fluxes from fluorescence lifetime imaging

X Yang, G Ha, DJ Needleman - Elife, 2021 - elifesciences.org
… We next considered a redox cycle between NADH and NAD+ with multiple oxidases and …
with 10 mM glucose. FLIM imaging revealed an increase of mitochondrial NAD(P)H intensity (…

[HTML][HTML] Metabolic response of Insulinoma 1E cells to glucose stimulation studied by fluorescence lifetime imaging

…, M Tesi, F Massarelli, L Marselli, P Marchetti… - FASEB …, 2020 - ncbi.nlm.nih.gov
… and activation of voltage‐sensitive calcium channels. Ca 2+ influx … the free and proteinbound
forms of NAD(P)H molecules. In … β‐cells to funnel glucose carbons from glycolysis to the …

Visualizing subcellular changes in the NAD (H) pool size versus redox state using fluorescence lifetime imaging microscopy of NADH

A Song, N Zhao, DC Hilpert, C Perry, JA Baur… - Communications …, 2024 - nature.com
… We show that NADH FLIM is sensitive to NAD(H) pool size, … impacting the protein-bound
NADH fluorescence lifetime, we … pyruvate, and 5 mM glucose and cell plates subsequently …

NAD (P) H autofluorescence lifetime imaging enables single cell analyses of cellular metabolism of osteoblasts in vitro and in vivo via two-photon microscopy

K Schilling, E Brown, X Zhang - Bone, 2022 - Elsevier
… In primary osteoblast culture, most glucose carbons are found to be incorporated into lactate
in … 4E&F) demonstrate the sensitivity of our current imaging approach to changes of energy …

Label-free characterization of single extracellular vesicles using two-photon fluorescence lifetime imaging microscopy of NAD (P) H

JE Sorrells, EM Martin, E Aksamitiene, P Mukherjee… - Scientific reports, 2021 - nature.com
protein-bound NADH has a fluorescence lifetime of approximately 1–3 ns and protein-bound
NADPH has a fluorescence lifetime … the fluorescence lifetime of NADPH bound to glucose-6-…

Using in vivo multiphoton fluorescence lifetime imaging to unravel disease-specific changes in the liver redox state

…, YH Mohammed, CA Thorling, H Wang… - … and Applications in …, 2020 - iopscience.iop.org
… destructive sampling to assess the fate of different drugs in liver … (free) form and in a protein
bound form, with these forms … , advanced liver fibrosis induced by carbon tetrachloride (CCl 4 )…

Fluorescence intensity and lifetime redox ratios detect metabolic perturbations in T cells

L Hu, N Wang, E Cardona, AJ Walsh - Biomedical Optics Express, 2020 - opg.optica.org
… redox ratio (FLIRR) based on the fractions of protein-bound NADH … are sensitive to metabolic
perturbations of T cells. FAD/… from tricarboxylic acid oxidation of glucose and β-oxidation of …

Development of fluorescence lifetime imaging microscopy techniques for analysis of single extracellular vesicles

JE Sorrells - 2020 - ideals.illinois.edu
… (FLIM) to characterize the free and protein-bound reduced forms of … by which glucose is
broken down into two threecarbonProtein-bound NAD(P)H lifetime is sensitive to multiple fates

Exploring tumor metabolism with time-resolved fluorescence methods: from single cells to a whole tumor

MV Shirmanova, VI Shcheslavskiy, MM Lukina… - … optical diagnostics of …, 2020 - Springer
… The fate of lactate secreted by glycolytic cancer cells remains to be … Glucose is a major,
but not the only fuel source for cancer … fluorescence lifetimes and ratio of free and protein-bound

Autofluorescence imaging to evaluate cellular metabolism

A Theodossiou, L Hu, N Wang, U Nguyen… - JoVE (Journal of …, 2021 - jove.com
… FDG-PET images the tissue uptake of FDG, a radiolabeled glucose analog. … Fluorescence
lifetimes are responsive to the … NAD(P)H has a shorter lifetime than protein-bound NAD(P)H