… no reference genes … genes can be used as reference genes to calibrate qPCR data of N.
tangutorum. In this study we analyzed the expression levels of 10 candidate reference genes (…

… Our research provided the best reference genes for RT-qPCR analysis of T. ciliata under
different conditions, laying a foundation for studying … for specific conditions using RT-PCR is …

… The lack of a systematic validation of reference genes: a serious pitfall undervalued in
reverse transcription‐polymerase chain reaction (RT‐PCR) analysis in plants. Plant …

… , 19 candidate reference genes were selected and … RT-PCR, and real-time quantitative PCR
(RT-qPCR). RT-qPCR has been widely used in molecular biology research, and expression …

… However, the use, selection and stability of reference genes are rarely verified in studies of
… In this study, we selected 13 candidate reference genes from the transcriptome data of G. …

… evaluation of 19 candidate reference genes. The expression levels of the 19 candidate
reference genes … However, the choice of the best HKGs for cancer studies remains dependent on …

… in the melting curve by RT-PCR. Furthermore, given that the … gel and a single peak in
RT-PCR observed for EF1, RPS18 … To validate the selected reference genes after determination …

… choice of reference genes, which are supposed to be stable across all samples. In this study,
we validated the expression … 12 candidate reference genes used in this study were selected …

… Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic
and … Identification and validation of reference genes for quantitative RT-PCR normalization in …

… expression patterns by selecting different reference genes … used and validated by many
research laboratories around the … conditions; (3) running RT-PCR and qRT-PCR. During this …