… Among these protein modifications, glycosylation is one of the most … Especially, we introduce
both semi-synthesis and chemical … N-glycan structure showed potent bioactivity in vivo. This …

… produced by human recombinant protein expression, which renders … synthetic glycosylated
EPO was evaluated using an in vitro … the semi-synthesis of a homogeneous glycosylated EPO …

… protein glycosylation systematically through a library of glycoproteins, each in homogeneous
… salicylaldehyde ester was coupled with a recombinant protein fragment (6-133) via Ser/Thr …

… fundamental aspects of homogeneous glycoprotein synthesis, … In vitro, the homogeneous
glycoform matched the potency of … glycoforms of EPO bearing BCTGs by recombinant protein …

… protein glycosylation. This review highlights the recent progress in the development of novel
strategies, preparation of homogeneous … coupled with a recombinant protein fragment (6—…

… Nature facilitates glycosylation of proteins heterogeneously such … ‘glycoform’, homogeneous
glycoproteins are essential. Often, … N-linked glycoproteins utilise recombinant proteins with N…

… In Chapter Three, the semi-synthesis of Interferon-γ is … tribulations associated with recombinant
protein expression, … It is clear that access to homogenous samples of glycosylated IFNγ …

… This approach utilizes a recombinant protein expression … to engineer homogeneous
well-defined protein-polymer … , 8 Protein semi-synthesis has expanded the scope of protein …

… protein glycoforms with various intact glycans. Here we report our synthesis and evaluation
of homogeneously glycosylated … N-glycosylation to the protein stability during in vitro folding, …

… to recombinant polypeptides. As a consequence, we succeeded in the semisynthesis of a
glycosylated form of … Therefore, the Cys modification strategy still has limitations in the selective …