… conducted with single myosin molecules, 61 myosins working … of 5.5 nm, generated during
a total step length of 9–13 nm. … 979 molecules (ie, from femtoNewtons to ~100 picoNewtons) …

… of an ensemble of myosin molecules either engineered or … of ~ 500 nm is imposed so that
the force drops to zero. The … rate of the relevant kinetic steps of the fast myosin isoform which …

… between mechanical and biochemical steps in the myosin–… ) of the myosin molecule (the
portion of the molecule containing … = 0 when the force in A1 is zero) ranging from −2.5 to 2.5 nm, …

… , generating piconewton force and nanometer displacement … , consisting in reduced force
and step size generated by … cofilament made of rods and myosin molecules [39]. However, the …

… myosin-5a molecules, displaying ~74 nm steps since only … to detect pico-Newton forces and
nanometer displacements … of the actin presented to the myosin molecules is increased. The …

… a tightly regulated process wherein the heart generates force to … Flow cells were sequentially
loaded with 200 nM myosin (wait 1 … detected from 10 myosin molecules for the unregulated …

… resolve the nanometre displacements and piconewton forces … the neck region of the myosin
molecule. The regulatory light … For a single myosin, the step size does not correspond to the …

… N = number of individual myosin molecules and n = number of … two steps in the bound events,
that is, δ1 = 4.37 ± 0.001 nm … -myosin lifetime of 0.046 s, the average stroke size of 4.04 nm …

… binding states during the force generating step must be … myosin motor domain of variable
lengths (∼8–12 nm long in … our understanding of force-generating step in myosin molecule. We …

… myosin molecules. This qualitative assessment of the data prompted a more robust examination
to determine the process … here were obtained with 10 nM GFP- tagged myosin- S1 (S1- …