Single-cell analysis of human retina identifies evolutionarily conserved and species-specific mechanisms controlling development

Y Lu, F Shiau, W Yi, S Lu, Q Wu, JD Pearson… - Developmental cell, 2020 - cell.com
… RNA sequencing (scRNA-seq) has allowed high-resolution analysis of … identify the
transcriptional networks governing human retinal development, we performed scRNA-seq analysis

[HTML][HTML] A single-cell guide to retinal development: Cell fate decisions of multipotent retinal progenitors in scRNA-seq

F Shiau, PA Ruzycki, BS Clark - Developmental biology, 2021 - Elsevier
… landscape governing organ development. Here, we review the application of single-cell
profiling techniques to the developing vertebrate retina, including early studies identifying

Gene regulatory networks controlling temporal patterning, neurogenesis, and cell-fate specification in mammalian retina

P Lyu, T Hoang, CP Santiago, ED Thomas, AE Timms… - Cell reports, 2021 - cell.com
… In this study, we use integrated single-cell RNA and single-cell ATAC sequencing (scATAC-…
Single-cell RNA-seq analysis of retinal development identifies NFI factors as regulating

Robust reprogramming of glia into neurons by inhibition of Notch signaling and NFI factors in adult mammalian retina

N Le, TD Vu, I Palazzo, R Pulya, Y Kim, S Blackshaw… - bioRxiv, 2023 - biorxiv.org
regulatory network that restores Müller glia to a resting state 6. Within this network, we identified
the Nuclear Factor I (NFI) … lineage analysis, immunohistochemistry, and single-cell RNA- …

Gene regulatory networks controlling vertebrate retinal regeneration

T Hoang, J Wang, P Boyd, F Wang, C Santiago, L Jiang… - Science, 2020 - science.org
… also conducted single-cell RNA-seq (scRNA-seq) to identify … well as after treatment with
exogenous factors that induced injury-… regulatory network that includes NFI factors, which are up-…

Nuclear Factor I in neurons, glia and during the formation of Müller glia‐derived progenitor cells in avian, porcine and primate retinas

HM El‐Hodiri, WA Campbell, LE Kelly… - Journal of …, 2022 - Wiley Online Library
… Chick single-cell RNAseq (scRNAseq) data are shared as … This study demonstrates that
NFI transcription factors are … RNA-Seq data for gene-cell matrices are deposited in GitHub …

Singlecell RNA sequencing: A new opportunity for retinal research

M You, R Rong, Z Zeng, H Li, X Xia… - Wiley Interdisciplinary …, 2021 - Wiley Online Library
… The mechanism by which NFI transcription factors regulate the … Moreover, it has identified
genes and transcription factors … Peng et al. used 165,000 single-cell RNA-seq profiles to …

[PDF][PDF] Robust reprogramming of glia into neurons by inhibition of Notch signaling and NFI factors in adult

N Le, TD Vu, I Palazzo, R Pulya, Y Kim, S Blackshaw… - Arbor - researchgate.net
regulatory network that restores Müller glia to a resting state 6. Within this network, we identified
the Nuclear Factor I (NFI) … lineage analysis, immunohistochemistry, and single-cell RNA- …

Single-cell analyses reveal transient retinal progenitor cells in the ciliary margin of developing human retina

B Dorgau, J Collin, A Rozanska, D Zerti… - Nature …, 2024 - nature.com
… In retinal progenitor cells, we identified a significant enrichment … Enrichment of NFI binding
motif family members was observed … Using spatiotemporal single cell RNA-Seq analyses we …

Single cell transcriptomics reveals lineage trajectory of retinal ganglion cells in wild-type and Atoh7-null retinas

F Wu, JE Bard, J Kann, D Yergeau, D Sapkota… - Nature …, 2021 - nature.com
… Here we report our further study of the roles of these transcription factors. Using bulk RNA-seq,
we identify genes regulated by the three transcription factors, which expand our …