… to one year significantly affected telomere shortening, which as … other sources of explants for
SE induction in pines. A recent … have shown that cold storage of mature embryos before their …

… Cell line and germination treatment significantly affected the shoot … on Scots pine somatic
embryos following maturation (before cold storage) and again after six to seven months of cold …

… The somatic embryos arose as single structures on explants, … of embryogenic tissue formation
was not profoundly affected … be improved by using a cold treatment, as demonstrated in …

… We investigated the effects of the explant sterilization method, … the possibility of considering
a cold preconditioning of plant … In Scots pine SEs, the size of the embryo increased when …

… initiation of this process in Scots pine (Pinus sylvestris L.), many … However, it was revealed
in our study that the explants from … medium affects the development of the embryonic mass [13,…

… that physicochemical conditions of one step may affect results of its subsequent steps. While
… We used mature zygotic embryos as explants, which may reduce the efficiency of spruce SE …

… , ie, somatic embryogenesis (SE) of Scots pine genotypes with … wood tissue [22], effects of
mechanical excision of explants … Samples (20–40 mg) were homogenized in 600 μL of ice-cold …

… somatic embryogenesis of representatives of the genus Larix. Particular attention is paid to
the choice of explants for somatic embryogenesis… the medium negatively affects the culture in …

… spruce by cryo-treating the explants with liquid nitrogen. This … with WIN1 in plant defense
against cold stress [50,51], which … , as epigenetic factors, critically affect the regulation of gene …

… somatic embryogenesis. Therefore, exploring the biological factors that affect somatic
embryogenesis … was homogenized in liquid nitrogen and extracted in cold 80% (v/v) methanol with …