[HTML][HTML] Rapid identification of pathogens involved in pediatric osteoarticular infections by multiplex PCR

C Gan, J Hu, Q Cao, R Zhao, Y Li, Z Wang… - Annals of …, 2020 - ncbi.nlm.nih.gov
… and mecA for methicillin-resistant Staphylococcus aureus, … The multiplex PCR was first
evaluated with 39 isolated clinical … multiplex PCR assay was designed to simultaneously detect

Triplex PCR combined with magnetic separation strategy for rapid and specific detection of methicillin-resistant Staphylococcus aureus in hospital samples

Z Wang, G Xie, G Chen, X Gao, J Li, Z Xie, H Xu - Microchemical Journal, 2021 - Elsevier
detection of pathogens is polymerase chain reaction (PCR)[18]. The multiplex PCR (mPCR)
developed in this study can detect … culture method, the detection efficiency was improved. …

… -Based biosensor coupled with multiplex loop-mediated isothermal amplification for detection of Staphylococcus aureus and identification of methicillin-resistant S …

L Jiang, X Li, R Gu, D Mu - Infection and Drug Resistance, 2020 - Taylor & Francis
Methicillin-resistant Staphylococcus aureus(MRSA) infection has become a serious worldwide
problem, with high mortality and morbidity since the 1970s. Citation3 As it is reported, in …

[HTML][HTML] … values of multiplex loop-mediated isothermal amplification and multiplex polymerase chain reaction for detection of methicillin-resistant Staphylococcus …

S Kashani, AH Alvandi, R Abiri - Jundishapur Journal of Microbiology, 2020 - brieflands.com
… develop detection assays for Staphylococcal mecA and spa using multiplex PCR and LAMP.
… aureus were confirmed to the species level using biochemical tests and multiplex PCR and …

The rapid and visual detection of methicillin-susceptible and methicillin-resistant Staphylococcus aureus using multiplex loop-mediated isothermal amplification …

X Chen, K Ma, X Yi, L Xiong, Y Wang, S Li - Antimicrobial Resistance & …, 2020 - Springer
… In recent decades, many molecular methods, such as polymerase chain reaction (PCR),
multiplex PCR and real-time PCR, have been applied for detecting MSSA and MRSA isolates […

[HTML][HTML] Comparison of PCR and phenotypic methods for the detection of methicillin resistant Staphylococcus aureus

A Madhavan, A Sachu, A Balakrishnan… - Iranian Journal of …, 2021 - ncbi.nlm.nih.gov
… Resistance to methicillin in methicillin resistant strains of Staphylococcus aureus (MRSA)
is due to the presence of mec-A gene, which encodes a low affinity penicillin binding protein (…

Value of multiplex PCR for detection of antimicrobial resistance in samples retrieved from patients with orthopaedic infections

IK Sigmund, N Renz, S Feihl, C Morgenstern, S Cabric… - BMC microbiology, 2020 - Springer
… the first generation of the multiplex PCR device reported a low detection rate (6%) of mecA
and mecC genes in methicillin-resistant staphylococci (not specified which subspecies), …

Identification of Methicillin-Resistant Staphylococcus aureus in poultry meat portions using Multiplex PCR

M Jafarzadeh, N Meamar, M Mohsenzadeh… - Journal of Poultry …, 2023 - jpsad.com
… This study investigates methicillin-resistant S. aureus (MRSA) isolates in poultry meat …
methicillin-resistant Staphylococcus aureus isolates from poultry meat portions by Multiplex PCR

Genetic Profiling of Methicillin-resistant Staphylococcus aureus in An African Hospital by Multiplex-PCR

SS Adeiza, JA Onaolapo… - Journal of Microbiology …, 2020 - dergipark.org.tr
… Further confirmation of identity was performed by multiplex PCR of the 16SrRNA, mecA,
tetM, pvl, nuc, spa and scn genes. DNA gel fingerprint analysis was carried out and the bands …

Novel species-specific targets for real-time PCR detection of four common pathogenic Staphylococcus spp.

B Zhou, Q Ye, M Chen, F Li, X Xiang, Y Shang, C Wang… - Food Control, 2022 - Elsevier
… , general PCR-based methods include multiplex PCR and multiplex quantitative PCR (qPCR) …
Based on the targets, we developed a qPCR technique for the rapid and specific detection