Novel species-specific targets for real-time PCR detection of four common pathogenic Staphylococcus spp.

B Zhou, Q Ye, M Chen, F Li, X Xiang, Y Shang, C Wang… - Food Control, 2022 - Elsevier
… , general PCR-based methods include multiplex PCR and multiplex quantitative PCR (qPCR) …
Based on the targets, we developed a qPCR technique for the rapid and specific detection

A Novel Assay for Detection of Methicillin-Resistant Staphylococcus aureus Directly From Clinical Samples

JA McClure, JM Conly, O Obasuyi, L Ward… - Frontiers in …, 2020 - frontiersin.org
… The timely detection of Methicillin-resistant Staphylococcus aureus (MRSA) is crucial for
antimicrobial therapy and a key factor to limit the hospital spread of MRSA. Currently available …

… of staphylococcal cassette chromosome mec in Staphylococcus aureus and non-aureus staphylococci from dairy cattle in Belgium: Comparison of multiplex PCR and …

CN Tchamba, F Touzain, M Fergestad… - Research in Veterinary …, 2023 - Elsevier
… The aim of this work was therefore to compare multiplex PCR (mPCR) and Whole Genome
Sequencing (WGS) to identify the SCCmec present in mec gene-positive, methicillin-resistant

[PDF][PDF] Validation of a Multiplex PCR for the Identification of Methicillin Resistant S. aureus from Uncultured Clinical Specimens

R Chikkala, P Saxena, KS Ratnakar, R Iyer, V Sritharan - gene - academia.edu
… We demonstrate that a multiplex PCR targeting nuc, mecA and 16s rRNA is accurate and …
the identification of MRSA, with a simple sample processing protocol for PCR based diagnosis

Development and application of a triplex‐PCR assay for rapid detection of methicillinresistant Staphylococcus aureus from pigs

S Rajkhowa, SR Pegu, GP Patil… - Letters in Applied …, 2021 - academic.oup.com
… A triplex‐PCR assay was developed and evaluated for rapid detection of methicillinresistant
Staphylococcus aureus (MRSA) recovered from various biological samples of pig. Three …

Development of a multiplex polymerase chain reaction-based DNA lateral flow assay as a point-of-care diagnostic for fast and simultaneous detection of MRSA and …

MT Kashef, OM Helmy - Diagnostics, 2022 - mdpi.com
… treatment of methicillin-resistant Staphylococcus aureus (… an MBDLFA capable of the specific
detection of S. aureus, … of the of the multiplex polymerase chain reaction (PCR)-based DNA …

[PDF][PDF] A simple and rapid molecular detection of Staphylococcus aureus strain B using multiplex PCR (P. 13)

F Sheikhi, M Zeinoddini, AS Nemati… - Chulalongkorn Medical …, 2020 - digital.car.chula.ac.th
… In this study, molecular detection methods such as polymerase chain reaction (PCR) and …
Specific detection of methicillinresistant Staphylococcus species by multiplex PCR. J Clin …

High prevalence of multi-drug resistant methicillin-resistant Staphylococcus aureus in tertiary Egyptian Hospitals

AAE Alfeky, MM Tawfick, MS Ashour… - The Journal of Infection in …, 2022 - jidc.org
… Thus, β-lactams are not the drugs of choice for the treatment of infections caused by
methicillin-resistant members of the Staphylococcus species [6]. Thirteen different types of SCCmec (…

[PDF][PDF] Multiplex PCR based detection of mecA, mecC and PVL gene in analysis of prevalence, circulation, transmission of MSSA/MRSA strains in a tertiary care …

SHS Meharaj, S Jayanthi, D Danisvijay… - … International Journal of …, 2023 - academia.edu
… mecA specific PCR does not routinely detect the new mecA homologue which is designated
… and prevalence of mecC associated methicillin-resistant Staphylococcus aureus (MRSA) …

[PDF][PDF] Standardization of multiplex PCR for identification of ten staphylococci species and their prevalence in bovine mastitis

TA Shafi, BK Bansal, S Sharma, NS Sharma - Indian J Anim Res, 2022 - researchgate.net
Methicillin-resistant Staphylococcus aureus (MRSA) is associated with low withinherd
prevalence of intra-mammary infections in dairy cows: Genotyping of isolates. Veterinary …