… data normalization, 18s rRNA was used as an internal control… A flower with the good and
healthy condition was selected … The qRT-PCR assay was optimized by running a series of …

… All expression level was normalized by samples. (d) The … , we conducted qRT-PCR analyses
of two selected genes at … -regulated gene pannel suitable for fiber improvement by CRISPR. …

… Through this study, six potential candidate internal control genes from Gossypium arboreum
… Suitable internal control genes for qRTPCR normalization in cotton fiber development and …

… ) genes in cotton fiber development need be further explored. … in the overexpression lines
according to qRT-PCR analysis; for … hirsutum samples were normalized by calibrating with the …

… GhUBI1 was used as an internal control for normalization. (B) … of fiber elongation and SCW
synthesis to form the appropriate … of cotton genes was analyzed by qRT-PCR using a cotton …

… Identifying cotton fiber development-related genes and … was used for relative gene
expression normalization 24 . The … to the qRT-PCR and RNA-seq data, we found that the gene …

… (PEPc, XTH and GA-20 Oxidase) transcript expression was determined by qRT-PCR (BioRad
iCycler iQ5). The expression levels were normalized with an internal control ubiquitin. The …

… ranked as the least stable genes in pooled samples. Pairwise … housekeeping genes would
be adequate to normalize the … of the suitable candidate’s HKGs for accurate qRT-PCR data …

… to 0.9, we finally obtain an appropriate soft-threshold value of 14, … levels determined by
qRT-PCR were normalized to the … effectors, reporter and internal control vectors used in transient …

… design qRT-PCR-specific primers, using the Upland cotton actin gene as a control for
normalization … In this study, four genes related to cotton fiber quality development were identified …