CST–Polα/Primase: the second telomere maintenance machine

SW Cai, T de Lange - Genes & Development, 2023 - genesdev.cshlp.org
… affecting the telomerase-mediated telomere maintenance pathway, … and requires CST–Polα/Primase-mediated
fill-in. (… In vitro, CST–Polα/Primase does initiate with a major RNA product, …

[HTML][HTML] Distinct functions of POT1 proteins contribute to the regulation of telomerase recruitment to telomeres

P Gu, S Jia, T Takasugi, VM Tesmer… - Nature …, 2021 - nature.com
… as substrates for telomerase-mediated telomere elongation. … to promote C-strand fill-in,
POT1b is also required to promote … (mTERT) in vitro and localizes to mouse telomeres in the …

[HTML][HTML] Reconstitution of a telomeric replicon organized by CST

AJ Zaug, KJ Goodrich, JJ Song, AE Sullivan, TR Cech - Nature, 2022 - nature.com
… This replication requires telomerase 3 extension of the single-stranded DNA (ssDNA) of the
… in vivo, where the products made by Polα–primase are transferred to DNA polymerase 𝛿 for …

ATR blocks telomerase from converting DNA breaks into telomeres

CG Kinzig, G Zakusilo, KK Takai, LR Myler, T de Lange - Science, 2024 - science.org
… Because telomerase requires a 3′ overhang in vitro (26), we anticipated that neotelomere
formation would require the MRN (Mre11/Rad50/Nbs1)/CtIP–mediated endonucleolytic …

[HTML][HTML] Telomere replication: solving multiple end replication problems

E Bonnell, E Pasquier, RJ Wellinger - Frontiers in Cell and …, 2021 - frontiersin.org
… Using in vitro conditions close to physiological states, it has … stimulation of the DNA Pol
αprimase complex facilitates the fork … complications due to telomerase-mediated over elongation …

CST does not evict elongating telomerase but prevents initiation by ssDNA binding

AJ Zaug, CJ Lim, CL Olson, MT Carilli… - Nucleic Acids …, 2021 - academic.oup.com
Pol α-primase binding appears to be required for nuclear … of the telomeric G-strand to pol
α-primase synthesis of the C-… from elongating DNA under multiple in vitro conditions, the switch …

[HTML][HTML] When the ends justify the means: regulation of telomere addition at double-strand breaks in yeast

RE Hoerr, K Ngo, KL Friedman - Frontiers in Cell and Developmental …, 2021 - frontiersin.org
… Neither group directly assessed the capacity of Cdc13 to bind the seed sequence in vitro,
so … is required for non-homologous end joining, promotes dnTA through interaction with the …

[HTML][HTML] Composition and function of telomerase—a polymerase associated with the origin of eukaryotes

PP Schrumpfová, J Fajkus - Biomolecules, 2020 - mdpi.com
… The maintenance of telomeres requires telomerase—a specific RNA-dependent … plant
extracts in vitro, did not always correlate with the ability to restore telomere maintenance in planta. …

Reconstitution of a telomeric replicon organized by CST

T Cech, A Zaug - 2021 - researchsquare.com
… Diede, SJ & Gottschling, DE Telomerase-mediated telomere addition in vivo requires DNA
primase and DNA polymerases … In vitro expansion of mammalian telomere repeats by DNA

Interstitial telomere sequences disrupt break-induced replication and drive formation of ectopic telomeres

EA Stivison, KJ Young, LS Symington - Nucleic acids research, 2020 - academic.oup.com
… subunit of the DNA polymerase delta (Pol δ) complex that … been shown to dismantle D-loops
in vitro and in vivo (66,67). … We also find that Mph1 is required to form ectopic telomeres. …