ArdA Protein Specificity against Type I Restriction–Modification Systems
AA Kudryavtseva, AV Vlasov, EV Zinovev… - Molecular Biology, 2024 - Springer
… The ArdA DNA-mimic antirestriction proteins inhibit type I … The ArdA specificity to DNA
methylation sites recognized by … of the EcoKI RMI system with a dimer of the antirestriction protein …
methylation sites recognized by … of the EcoKI RMI system with a dimer of the antirestriction protein …
[HTML][HTML] Functional comparison of anti-restriction and anti-methylation activities of ArdA, KlcA, and KlcAHS from Klebsiella pneumoniae
H Chen, S Tao, N Li, F Wang, L Wang… - Frontiers in Cellular …, 2022 - frontiersin.org
… The anti-restriction protein ArdA belongs to the DNA mimic … In other words, DNA methylation
protects it from the restrictive … of the type I RM enzyme EcoKI, indicating that the KlcA protein …
protects it from the restrictive … of the type I RM enzyme EcoKI, indicating that the KlcA protein …
Restriction-modification systems as mobile epigenetic elements
Y Furuta, I Kobayashi - Bacterial integrative mobile genetic …, 2022 - taylorfrancis.com
… A second copy of S gene is on a plasmid. D) … of epigenetic DNA methylation decreases,
while this Type IV system … evolved anti-restriction features, such as solitary methyltransferases …
while this Type IV system … evolved anti-restriction features, such as solitary methyltransferases …
[HTML][HTML] Bacterial DNA methyltransferase: A key to the epigenetic world with lessons learned from proteobacteria
…, L He, M Wang, R Jia, S Chen, D Zhu, M Liu… - Frontiers in …, 2023 - frontiersin.org
… proposed that epigenetic memory systems have numerous potential applications in synthetic
biology, including life biosensors, death switches or induction systems for industrial protein …
biology, including life biosensors, death switches or induction systems for industrial protein …
Phage T7 DNA mimic protein Ocr is a potent inhibitor of BREX defence
A Isaev, A Drobiazko, N Sierro, J Gordeeva… - Nucleic acids …, 2020 - academic.oup.com
… several other DNA mimic antirestriction proteins, overcomes … coli AB1157 carrying a genomic
copy of type I EcoKI R–M … binds to BrxX, a DNA methyltransferase, which is responsible for …
copy of type I EcoKI R–M … binds to BrxX, a DNA methyltransferase, which is responsible for …
RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation
M Skutel, D Yanovskaya, A Demkina… - Nucleic acids …, 2024 - academic.oup.com
… ArdA is a DNA mimic (46); ArdB inhibits Type IR–M in vivo … on AB1157ΔhsdM and thus
lacked EcoKI methylation. … this EcoKI site is required for anti-restriction activity, we introduced …
lacked EcoKI methylation. … this EcoKI site is required for anti-restriction activity, we introduced …
Plasmid replication-associated single-strand-specific methyltransferases
A Fomenkov, Z Sun, IA Murray, C Ruse… - Nucleic Acids …, 2020 - academic.oup.com
… was used to investigate DNA methyltransferases M.BceJIII and … Thus, we can separate
copy-number effects from effects of … MG1655, and likely expresses the Type I enzyme EcoKI. …
copy-number effects from effects of … MG1655, and likely expresses the Type I enzyme EcoKI. …
Structural features of a minimal intact methyltransferase of a type I restriction-modification system
PW Seo, A Hofmann, JH Kim, SA Hwangbo… - International Journal of …, 2022 - Elsevier
… with the DNA-mimicking anti-restriction proteins Ocr and … the M-domain adds further structural
information and improves understanding of the mechanism of DNA methylation by the type …
information and improves understanding of the mechanism of DNA methylation by the type …
[HTML][HTML] N6-methyladenine and epigenetic immunity of Deinococcus radiodurans
… DNA methylation is ubiquitously found in all three domains of … of this ORF to mimic synthetic
construct DraRI reported earlier [… Another anti-restriction strategy is restriction site avoidance, …
construct DraRI reported earlier [… Another anti-restriction strategy is restriction site avoidance, …
Low-level expression of the Type II restriction–modification system confers potent bacteriophage resistance in Escherichia coli
…, I Mruk, B Furmanek-Blaszk, M Sektas - DNA …, 2020 - academic.oup.com
… ) and a DNA methyltransferase (MTase). Most entering … levels of the R–M proteins were
associated with reduced … activities from a single-copy R–M system is operating very close to the …
associated with reduced … activities from a single-copy R–M system is operating very close to the …