Characterization of Testicular and Non-Testicular Transcripts of Human and Mice Phospholipase C zeta

SEA Elrasheed - 2022 - search.proquest.com
… This study screened mouse and human tissues for PLCζ … impact on gene expression profile
during early embryogenesis (… isolated from Testis and epididymis WT, and testis homozygous …

Identification and Characterisation of Novel Isoforms of PLCζ in Non-testicular Tissue and Cells

NSS Gaber - 2022 - search.proquest.com
… that the XY-linker region in mouse PLCζ comprises a nuclear … To detect PLCζ protein in
epididymal and other cell lines, EF … in mouse and human does not support its role as a sperm-…

Advancing male age differentially alters levels and localization patterns of PLCzeta in sperm and testes from different mouse strains

J Kashir, BV Mistry, MA Gumssani… - Asian Journal of …, 2021 - journals.lww.com
… age in human males did not significantly alter sperm PLCζ, although … ) also affected PLCζ
profiles in mouse sperm and testes. … allow quantification of tail fluorescence. However, as the …

Essential role of sperm-specific PLC-zeta in egg activation and male factor infertility: an update

A Saleh, J Kashir, A Thanassoulas… - Frontiers in cell and …, 2020 - frontiersin.org
… In the mammalian testis, PLCζ expression is detected at … PLCζ polyclonal antibodies
against human, mouse, and porcine … This work was supported by the Qatar University student …

Production of mouse offspring from inactivated spermatozoa using horse PLCζ mRNA

Y Yamamoto, N Hirose, S Kamimura… - … of Reproduction and …, 2020 - jstage.jst.go.jp
… medium containing 0.1% bovine testicular hyaluronidase for 3 min. … Epididymides were
removed from male ICR mice. After both … Previously, recombinant human PLCζ or porcine PLCζ

Evaluating the Applicability of PLCζ as a Diagnostic Tool in Correlation to Sperm Health

RB Abu-Dawas - 2022 - search.proquest.com
… We examined PLCζ expression and localization in different parts of mice epididymis sperm
… that the PLCζ was localized in the testis since its expression was significantly reduced in the …

The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice

J Kashir, BV Mistry, MA Rajab, L BuSaleh… - Human …, 2024 - academic.oup.com
… mutant Plcζ mouse experiments do suggest that PLCζ plays … sperm and epididymal quality
of such mutant mice. However, … analyses were performed on testes and epididymides from a …

PLCZ1 deficiency decreased fertility in male mice which is associated with sperm quality decline and abnormal cytoskeleton in epididymis

T Wang, B Cao, Y Cai, S Chen, B Wang… - International Journal of …, 2022 - mdpi.com
… of Plcz1 m5 mice at 48 h post-human chorionic gonadotropin (… (D) Detection of PLCζ protein
expression in the testes of … analysis of z-scored expression profiles of transcripts that were up…

Functions and gene expression alterations of phospholipase C in gametes

M Yeste, A Delgado-Bermúdez, C Jones… - … in Physiology and …, 2023 - Elsevier
… When microinjected into mouse oocytes, human PLCζ is more able than its mouse
testicular and epididymal expression profile of PLCζ in mouse and human does not support

Evaluation and Characterization of PLCzeta in the Male Reproductive Tract

RA Alshahrani - 2022 - search.proquest.com
… PLC zeta expression done in transgenic mice testis using RNA … on transgenic mice to describe
the generation of PLCζ- null … Following sacrificing, mouse Cauda epididymis was isolated …