Transcription modulates chromatin dynamics and locus configuration sampling

G Forte, A Buckle, S Boyle, D Marenduzzo… - Nature Structural & …, 2023 - nature.com
… For this, we discriminate between two chromatin states, one … strongly on the size of the
chromatin segment being tracked. … real-time quantitative reverse transcription PCR (qRT–PCR); …

Improving the Efficiency of Early Cancer Detection using Single-Cell ATAC-seq Data for Internet of Biomedical Things

H Haririmonfared, N Elmi, K Kavousi… - 2024 10th International …, 2024 - ieeexplore.ieee.org
… the way for close to real-time cancer risk assessment via the … diagnosis but also allow for
real-time monitoring of patients' vital … • Elucidating the chromatin state in schizophrenia patients …

[HTML][HTML] Breaking boundaries: Pan BETi disrupt 3D chromatin structure, BD2-selective BETi are strictly epigenetic transcriptional regulators

LM Tsujikawa, OA Kharenko, SC Stotz… - Biomedicine & …, 2022 - Elsevier
Real-time PCR was used to determine abundance of the transcript relative to the endogenous
control cyclophilin in the same sample using the … Green bars at the top of the track indicate …

Global mapping of RNA-chromatin contacts reveals a proximity-dominated connectivity model for ncRNA-gene interactions

C Limouse, OK Smith, D Jukam, KA Fryer… - Nature …, 2023 - nature.com
… The gray track shows the predicted interaction profile based … Understanding how caRNAs
control chromatin state and … cycled on an ABI ViiA 7 Real-Time PCR Machine with the following …

[HTML][HTML] From donor to the lab: a fascinating journey of primary cell lines

M Richter, O Piwocka, M Musielak… - Frontiers in Cell and …, 2021 - frontiersin.org
… , dispase, and DNase), chemical treatment (with ethylene … , flow cytometry, real-time polymerase
chain reaction, karyotyping, … designing 3D tumours and tracking cell migration, however, …

Massively multiplex single-molecule oligonucleosome footprinting

NJ Abdulhay, CP McNally, LJ Hsieh, S Kasinathan… - Elife, 2020 - elifesciences.org
real-time sequencing could detect m6dA deposited on … depleted for each chromatin state.
Tests qualitatively appearing to be … We also kept track of the molecules where find_peaks could …

ZNF148 inhibits HBV replication by downregulating RXRα transcription

X Yao, K Xu, N Tao, S Cheng, H Chen, D Zhang… - Virology Journal, 2024 - Springer
… Northern blotting and real-time PCR were used to quantify the … , and the supernatant was
incubated with DNase I and MgCl 2 at … chromatin was analyzed by real-time PCR. GAPDH was …

The zinc finger protein CLAMP promotes long-range chromatin interactions that mediate dosage compensation of the Drosophila male X-chromosome

W Jordan III, E Larschan - Epigenetics & Chromatin, 2021 - Springer
… even when restricting our analysis to only active chromatin regions as controls (p = 5.7e−107…
identified after msl2 RNAi and we find similar chromatin states to those identified at CLAMP-…

Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interacting RNA

M Baker, R Khosravi, M Salton - RNA-Protein Complexes and Interactions …, 2023 - Springer
… of a protein followed by monitoring the RNA molecules that bind to it. … RIP by the use of
semi-qPCR or real-time polymerase chain reaction (… Make sure to use a kit with DNase treatment. …

An intronic enhancer of Cebpa regulates adipocyte differentiation and adipose tissue development via long‐range loop formation

X Li, S Zeng, L Chen, Y Zhang, X Li, B Zhang… - Cell …, 2024 - Wiley Online Library
… the template for quantitative real-time PCR (qRT-PCR). qRT-… (A) Upper track shows the
circularized chromosome … Our findings suggest that the chromatin state and chromatin