… For this, we discriminate between two chromatin states, one … strongly on the size of the
chromatin segment being tracked. … real-time quantitative reverse transcription PCR (qRT–PCR); …

… /Taz in regulating chromatin state, we administered doxycycline … Quantitative real-time PCR
(qRT-PCR) was completed using … used to visualize BigWig files on the mm10 genome track. …

… In this study, we track the fate of centromeres during aging in … across the various chromatin
states (euchromatin and … (D) Quantitative real-time PCR for centromeric transcripts using …

… Other chromatin states increased DNA methylation levels … The repetitive regions were obtained
from the track “RepeatMasker,” … TO performed quantitative real-time PCR and helped with …

… real-time sequencing could detect m6dA deposited on … depleted for each chromatin state.
Tests qualitatively appearing to be … We also kept track of the molecules where find_peaks could …

… of a protein followed by monitoring the RNA molecules that bind to it. … RIP by the use of
semi-qPCR or real-time polymerase chain reaction (… Make sure to use a kit with DNase treatment. …

… Chromatin states associated with DNA methylation were also … histone modifications were
prepared using controls for two histone … was learned using LearnModel for 18 chromatin states, …

… using six histone modifications to define ten chromatin states … (C) Track plot for each
CRISPRa perturbation. The gene … , 1 μg of RNA were treated with TURBOTM DNase (Thermo) …

… chromatin was performed as for the ChIP-seq for cells but with pulses increased to 4 for tissue.
Real-time … of IHH cells cultured in control or oleic acid-supplemented media after DNAse I …

… by monitoring the therapeutic effects of venous DNase I … C for 30 min to degrade chromatin.
The obtained protein mixtures were … in hemodialysis quantified by real-time PCR. Clin. Chem. …