… Escherichia coli transcription initiates with RNAP holoenzyme, ie, core enzyme plus a sigma
… In this study, we used single-molecule fluorescence measurements to primarily examine the …

… The Altmetric Attention Score is a quantitative measure of the … binding, DNA-bending
transcription activators are discussed. … DNA occurs before the release of downstream contacts. …

… of naturally occurring transcriptional and translational … released anti-terminator sequence
sequesters the terminator … intensities measured with the transcriptional activator in the …

… sequences to terminate transcription of the Escherichia coli RNA … coli cells for measuring
transcription termination by … They were then placed at 75C for 10 min to inactivate the enzyme. …

… In this study, we measured the location of the 3’ ends of the galET mRNA more precisely …
endoribonuclease, can process the 3’ end of a transcript that is released from RDT (Fig 5). It is …

… in the sequence or size of the transcription bubble trigger global … by DNA-dependent RNA
polymerases (RNAPs) releases … new modes of transcription initiation (4) and termination (5, 6), …

… focuses on mechanisms specific to Escherichia coli σ 70 (6); … RNA transcripts are repeatedly
generated and released (50… a promoter’s association rate (k on ) measured in vitro to in vivo …

… Taking the time of probe release as the time of termination, we measured the fluorescence …
, MJ Transcription termination in Escherichia coli. Measurement of the rate of enzyme release …

… By monitoring Escherichia coli nascent riboswitches, we reveal … (E FRET ∼0.8) was measured
for EC-83 nascent transcripts, as … when using methods based on enzymatic ligation (57). …

… For example, metabolite concentration measurements made … more than one enzyme
targeted by end-product inhibition, … measured expression without accounting for transcriptional …