[HTML][HTML] Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML

M Marchesini, A Gherli, E Simoncini, LMD Tor… - Nature …, 2024 - nature.com
… The overexpression of the ecotropic viral integration site-1 gene (… expression in TF1 cells
6 days after shRNA transduction. D … stem cells (HSCs) (61.4%) and lympho-myeloid primed …

Targeting leukemic stem cells in a murine model of CALM-AF10 positive acute myeloid leukemia

T Mandal - 2021 - oparu.uni-ulm.de
… -generation retrovirus producer cell line for the generation of helper-free ecotropic retroviruses.
HEK-293T … In cases where viral titers of the transduced GP+E86 fibroblasts bulk cell

Optogenetic and multiplexed gene editing in primary T-cells.

D Lake - 2023 - diva-portal.org
… through transducing T-cells with the mouse stem cell virus (… was able to transduce human
embryonic kidney cells (HEK293) … cells which are effective at packing eco-tropic retrovirus

Human Cell Line Panel With Human/Mouse Artificial Chromosomes for Functional Analyses of Desired Genes

N Uno, S Takata, S Komoto, H Miyamoto, Y Nakayaka… - 2021 - researchsquare.com
… employed mouse embryonic stem cells that contain a MAC … -term cell culture, which differed
from gene transduction with … activity in MSCs with a viral vector system for gene expression …

[HTML][HTML] The ETV6-MECOM fusion protein promotes EMT-related properties by repressing the transactivation activity of E-cadherin promoter in K562 leukemia cells

Q Li, F Wang, X Zhang, S Liu, MZ Sun, J Yan - Biochemistry and Biophysics …, 2024 - Elsevier
… syndrome 1 (MDS1) and ecotropic viral integration site 1 (EVI1… blot (B) results of K562 cells
stably transduced with the pLVX-… -renewal ability of leukemia stem cells and delays leukemia …

[HTML][HTML] Mouse strain-specific polymorphic provirus functions as cis-regulatory element leading to epigenomic and transcriptomic variations

X Zhou, TW Sam, AY Lee, D Leung - Nature Communications, 2021 - nature.com
… ERVs, we analyze murine embryonic stem cells (mESCs) from … for cells transduced with
each sgRNA is compared to vector only … d ChIP-qPCR of sgRNA-transduced and control cells

The impact of MEIS1 TALE homeodomain transcription factor knockdown on glioma stem cell growth

HJ Kim, DC Batara, YJ Jeon, S Lee… - Animal Cells and …, 2024 - Taylor & Francis
… Myeloid ecotropic virus insertion site 1 (MEIS1) is a HOX co-… in gliomagenesis particularly
on glioma stem cells (GSCs) … volume, GSC11 was transduced with a constitutive luciferase …

PHF6 suppresses self-renewal of leukemic stem cells in AML

SS Jalnapurkar, A Pawar, SS George, C Antony… - bioRxiv, 2024 - biorxiv.org
… We identify that LICs in the Hoxa9 transduction model are concentrated within … Retroviruses
were generated by transient co-transfection of 293T cells with retroviral vector and ecotropic

[HTML][HTML] VEGFR-2 redirected CAR-T cells are functionally impaired by soluble VEGF-A competition for receptor binding

E Lanitis, P Kosti, C Ronet, E Cribioli… - … for Immunotherapy of …, 2021 - ncbi.nlm.nih.gov
… The Phoenix Eco retroviral ecotropic packaging cell line, … As a control, a retroviral vector
pMSGV encoding the marker … on primary murine T cells following retroviral transduction. (C) …

MECOM promotes leukemia progression and inhibits mast cell differentiation through functional competition with GATA2

K Iida, M Nakanishi, J Nakahara, S Asada, T Isobe… - bioRxiv, 2024 - biorxiv.org
… downregulated in MECOM-WT-transduced CB cells compared to vector-transduced CB cells
… with the relative mRNA level in vector-transduced cells set to 1. Data are presented as 18 …