… retroviral vectors can only integrate into actively dividing cells… Interestingly, when we examined
the transduction efficiency … true both for murine embryonic stem cells (ESC) differentiating …

… of three human suspension 293-F-derived ecotropic MLV-… in six-well dishes 1 day prior to
transduction (Nunc, Wiesbaden, … Cell-free and viral vector-containing cell culture supernatant …

… These iPS-like cells expressed embryonic stem cell markers, … On the next day, the cells were
transduced with 3 μg of pLenti6/UbC/… an ecotropic retroviral receptor necessary for retroviral …

… for the efficient retroviral transduction and expansion of murine … We present a bicistronic
retroviral vector encoding both a tumor … The Phoenix Eco retroviral ecotropic packaging cell line, …

… by replication-defective viral vectors derived from the human immunodeficiency virus type 1
(… Thus, even when cells stably transduced by an HIV-1-based vector are transfected with the …

… Adenovirus vector is a kind of viral vector used often for gene … Embryonic stem cells (ESCs)
differentiate into … -10 (IL-10) through adenoviral transduction [10]. Transplantation of hypoxia-…

… Our mixed virus strategy yielded high dual transduction … We next built retroviral vectors
encoding the SiRPα monomers (… mouse ecotropic retroviral particles, Phoenix-ECO cells were …

… -transfect cells such as neurons and primary cells. In recent years, viral vectors have become
… However, ecotropic and GaLV viral titers at 72 h will be ~20–40% less than at 48 h. VGV-G …

… We show here that established human embryonic stem cells (hESCs) have stable telomere
… ecotropic packaging plasmid pCL-Ampho (4 μg) together with one of the following retroviral …

… Multipotent stem cells arise during embryonic development … to maintain haematopoiesis
throughout a human life. Defects in … a transduction status from left to right: Un-transduced, Vector, …