… Ecotropic viral integration site 1 (EVI1), one of several … Moreover, the key roles of this gene
in hematopoietic stem cells have … (B) CAL-33 cells were transduced with a retroviral vector …

… cell cultures, using polybrene (5 μg/ml) as transduction … new vector was transfected into
Phoenix ecotropic retroviral … The role of embryonic stem cells in cancer has been largely …

… adult life from some type of stem cell precursor, as they are in … An added important feature of
retroviral vectors is that the … ), it will be necessary to transduce all the cells for them to remain …

… HR technology performs well in mice embryonic stem cells (ESCs), while the unavailable
isolation of ESCs from livestock enforced scientists to conduct HR in somatic cells to produce …

… inhibits ecotropic MLV infection without affecting the cell … The VSV-pseudotyped retroviral
vectors are widely utilized … cells compared with that in the empty vector-transduced cells (Figure …

… of primary cells transduced with ORF, shRNA and CRISPR-Cas viral … activation of endogenous
ecotropic retroviruses gives rise to … Southern blots of retroviral vector integration and …

… a common ecotropic retroviral integration in the same locus, termed ecotropic viral integration
… are maintained by rare stem cells (Leukaemic stem cell), and that elimination of leukaemic …

… affinities for the same regions in embryonic stem cells (ESC). Also … cell lines transduced with
a pBabePuro retroviral vector. … TM vectors were transfected into the ecotropic packaging cell …

… blood cells derive from multipotent hematopoietic stem cells (… ecotropic viral integration site
1 homolog, Tal1/Scl = T-cell … -1 cells were utilized for evaluating the viral vector transducing …

… (which varied based on viral titers and transduction efficiency) … -ecotropic env-SV40 ori, were
transfected into HEK293T cells (… -based retroviral vectors was performed in HEK293T cells, …