[HTML][HTML] Multisite transformation in Neisseria gonorrhoeae: insights on transformations mechanisms and new genetic modification protocols

VY Seow, O Tsygelnytska, N Biais - Frontiers in Microbiology, 2023 - frontiersin.org
… Using Neisseria gonorrhoeae as a model system and using transformation with multiple DNA
molecules… of Neisseria gonorrhoeae rely largely on transforming the bacteria with genomic …

DNA Transformation and Type IV Pili in Neisseria gonorrhoeae

VY Seow - 2024 - theses.hal.science
… specifics of natural transformation remain elusive. Neisseria gonorrhoeae's dependence on
… the intricate processes of DNA transformation within Neisseria gonorrhoeae, responding to …

A Modular Plasmid System for Expression of Genes in Neisseria gonorrhoeae

CD Flemming - 2023 - researchcommons.waikato.ac.nz
… is a rarely used method for transformation of DNA into N. gonorrhoeae as it is far less
efficient, but can be useful when transforming a nonpiliated strain or when transforming with …

Markerless gene editing in Neisseria gonorrhoeae

RA Jones, WX Yee, K Mader, CM Tang… - …, 2022 - microbiologyresearch.org
… Unmarked gene deletions can be achieved by transforming N. gonorrhoeae with DNA
harbouring … To generate markerless deletions, we performed a second transformation with a PCR …

[HTML][HTML] Mobility of extracellular DNA within gonococcal colonies

N Bender, M Hennes, B Maier - Biofilm, 2022 - Elsevier
… of DNA within colonies formed by Neisseria gonorrhoeae, and relate it to transformation
be saturated with DNA that transforms but does not repair the point mutation within sfgfp. …

Neisseria gonorrhoeae physiology and pathogenesis

LR Green, J Cole, EFD Parga, JG Shaw - Advances in microbial physiology, 2022 - Elsevier
Neisseria spp. utilize a gene conversion mechanism whereby the expressed pilE locus,
the main constituent of the pilus filament, is replaced by a variant DNA sequence from a silent …

Evolution and exchange of plasmids in pathogenic Neisseria

WX Yee, T Elsener, A Cehovin, MCJ Maiden, CM Tang - Msphere, 2023 - Am Soc Microbiol
… A conjugative plasmid which is widespread in Neisseria gonorrhoeae, … tumefaciens,
VirB6, is thought to form a channel through which DNA … Products were used to transform N. …

[HTML][HTML] Gonococcal resistance to zoliflodacin could emerge via transformation from commensal Neisseria species. An in-vitro transformation study

S Abdellati, JGE Laumen, T de Block, I De Baetselier… - Scientific Reports, 2024 - nature.com
… bacterial DNA replication via interactions with DNA gyrase subunit B 2 . Studies have found
very little resistance to zoliflodacin in currently circulating strains of Neisseria gonorrhoeae 3 …

[HTML][HTML] Phase-variable Type I methyltransferase M.NgoAV from Neisseria gonorrhoeae FA1090 regulates phasevarion expression and gonococcal phenotype

…, P Bacal, A Mrozek, N Matczynska… - Frontiers in …, 2022 - frontiersin.org
Neisseria gonorrhoeae FA1090 and variant strains were grown on … gene was amplified
from purified chromosomal DNA of N. … results indicated that cloned MTase from transformed N. …

[HTML][HTML] Mechanisms of host manipulation by Neisseria gonorrhoeae

E Walker, S van Niekerk, K Hanning, W Kelton… - Frontiers in …, 2023 - frontiersin.org
… Cells in the female reproductive tract transition from non-… expression in cells found in the
transition zone. To date, there … CD105, a member of the transforming growth factor-β1 receptor …