… leveraged to perform targeted mutagenesis and disruption of … ACIAD0135, by transforming
an OE-PCR product containing the … in the strains we created could be combined in future work …

… be created through primers with polymerase chain reaction (PCR). The DNA … strain to
validate their causality. The process can be laborious, especially when there is a large mutation …

… created using the above natural transformation workflow. A single set of mutagenic primers
was … These insulators were added by successive PCR mutagenesis via overhangs to create …

… exceptional efficiency of natural transformation and allelic replacement in ADP1, an ideal …
This new strain, ADP1-ISx, demonstrated reduced mutation rates and higher transformation …

… Its high efficiency of natural transformation and allelic … by direct addition of the purified
PCR product to the exponentially … Although the mutation-transformed cells could be directly …

… For example, although we tout the plasticity of strain ADP1 and … carrying a known mutation
from the evolved strain, were … -transformation of different mutated alleles, each PCR product …

… , the natural competency of Acinetobacter baylyi ADP1 facilitated … a beneficial mutation
(white star). A second beneficial … of the GOI may occur during PCR or transformation. Therefore, …

… A PCR fragment containing the cat gene which conferred … introduced into XH440 by natural
transformation (Fig. 1a). The A. … The mutation rates of four strains were shown in Table 2 and …

… amplified by PCR and fused tail-to-head by overlap extension PCR (see … baylyi mutants
were naturally transformed with plasmids … Another mutation identified in the parent-strain IP148 …

… transformation as one of the paired cells can be transformed … the strains characteristic
frequency of natural transformation. … for the PCR are shown in Table 9 and the PCR program is …