… incorporating additional cell types and hormone signaling. … While they can be cultured for
extended periods, it is important to be … noncanonical Wnt signaling. Proceedings of the National …

… extend the potential of this approach to facilitate in situ 3D … and FGF signaling factors in
mammary organoid maintenance … , the organoids can maintain the hormone responsive cells, …

… and juxtacrine signaling in regulation of mammary architecture … To study mammary epithelial
cell behavior in response to … and milk production upon proper hormonal treatment, and an …

… stem cell (ISC) proliferation and is a key component of organoid … pathways that orchestrate
these decisions are the WNT, … the substantial regenerative response triggered upon epithelial …

… a substantial growth retardation (Extended Data Fig. 1c). The … /2 in the intestine by maintaining
WNT signalling 13,14 . We posit … The isolation and culture of mammary organoids were as …

… peritoneal fluid in the modulation of the WNT signaling and their relation with the development
and … The utilization of 3D cultures and organoids will help to predict the responsiveness of …

… established a mammary organoid system via co-culture of … , we tested the hormone
responsiveness of M2 co-cultured organoids. … Wnt signaling activation in fibroblasts is responsible …

… engagement of the canonical Wnt/β-catenin/TCF signaling pathway as well as … and 3D
mammary epithelial cell culture models display similar [Ca 2+ ] i signaling dynamics in response …

… culture approach based on 3D primary mammary epithelial … diverse culture conditions, such
as growth factors, hormones, … conditions allow to extend the organoid cultures to an average …

… of 3D culture of tumor cells and breast cancer organoids has … confirmed the heterogeneity
of organoid response to drugs. … Studies have shown that extended growth of these organoids…