… The recombinant Escherichia coli cells were constructed by co-expressing L-amino acid …
, α-keto acid decarboxylase from Proteus mirabilis, aldehyde reductase from Escherichia coli (…

… This study has developed a robust whole-cell E. coli biocatalyst for α-keto acids production
by protein engineering, and this strategy may be useful for the construction of other …

… In this study, we cloned three genes encoding the three KADC variants into Escherichia coli
and characterized the corresponding recombinant protein. Additionally, homology models of …

… the types and applications of keto acids, and then summarize … of typical keto acids, including
pyruvic acid, oxaloacetic acid, α-… α-Keto acids have the keto group adjacent to the carboxylic …

… via the asymmetric reduction of prochiral ketones by oxidoreductases under mild
conditions, … aldo–keto reductases (AKRs) and exhibit a strict substrate specificity toward α-ketoesters, …

… Production of natural α-keto acids was performed with 20 g L –1 of wet E. coli cells (containing …
Production of unnatural α-keto acids was performed in duplicate with 20 g L –1 of wet …

… stratosphericus LAMA 585 was synthesized as an Escherichia coli codon-optimized gene
and cloned into a pET-11a vector by GenScript. A–KR–PCP (residues 1–1,318) and the A …

… engineering of Escherichia coli and selected mutations of α-keto acid decarboxylase …
chain-extended α-ketoacids was introduced into Escherichia coli BL21 (DE3). Then, the extended α-…

… coli [15,16]. Both CPR-C1 and CPR-2 reduce α-diketoesters such as KPL to DPL in a
strictly stereospecific manner. CPRs are classified into the aldo-keto reductases (AKRs) …

… Nineteen mutants at each site were constructed and expressed in Escherichia coli (DE3).
The specific activity of all 57 variants of PmiLAAD was measured by the DNPH method …