… of genetic networks in mammalian cells is still painstakingly slow and fraught with
inexplicable failures. Here, we demonstrate that transiently expressed genes in mammalian cells …

… cell atlases of gene expression and chromatin accessibility in fetal tissues. For gene expression,
… cells. We leveraged the literature and other atlases to identify and annotate hundreds of …

… gene expression in mammalian cells is crucial for safe and efficacious gene therapies and for
elucidating gene … switch that controls mammalian gene expression through modulation of a …

RNA interference (RNAi) is mediated by an ∼21-nt double-stranded small interfering RNA (siRNA)
and shows great promise in delineating gene functions and in developing …

… Beginning with two alleles (two to four gene copies, depending on the cell cycle stage), a
typical gene will populate a mammalian cell with one to a few thousand transcripts. Several …

… expression systems affects the expression vector to be used. During transient gene expression
… into the genome of the cell and as result the transfected genes only express in a limited …

… Here we report a re-engineered version of the classic 10–23 DNAzyme that mediates persistent
gene-silencing activity in cultured mammalian cells and simultaneously resists nuclease …

… (A) Using parameters typical of mammalian cells, we find that our theory agrees well with
simulations for d / d p = 30 , is acceptable for d / d p = 10 , and performs poorly for d / d p = 5 ; …

… In agreement with this model, depletion of CCR4–NOT components in mammalian cells
results in an overall increase in mRNA half-lives but depletion of PAN2–PAN3 does not have a …

… signalling from gene expression measurements of bulk and single-cell data … expression of
their cognate genes. In this Review, we highlight discoveries enabled by analyses of cell–cell …