A novel spike subunit 1‐based enzyme‐linked immunosorbent assay reveals widespread porcine torovirus infection in eastern China

P Qin, YL Yang, ZM Hu, YQ Zhang… - Transboundary and …, 2022 - Wiley Online Library
… -positive faecal sample by RT-PCR amplification of overlapping regions spanning the entire
genome. Viral cDNAs were amplified by SuperScript II with random primers according to the …

Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses

Z Pan, J Lu, N Wang, WT He, L Zhang, W Zhao, S Su - Virulence, 2020 - Taylor & Francis
amplification curves represent samples positive for PEDV, PDCoV, PToV, and SADS-CoV
detected by our multiplex real-time PCR assay… of coronavirus and torovirus, we cannot …

Emergent molecular techniques applied to the detection of porcine viruses

EA Flores-Contreras, JA Carrasco-González… - Veterinary …, 2023 - mdpi.com
swine industry are described in this review, including African swine fever virus (ASFV),
porcine … virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and porcine circovirus (PCV), …

Characterization of self-processing activities and substrate specificities of porcine torovirus 3C-like protease

S Xu, J Zhou, Y Chen, X Tong, Z Wang, J Guo… - Journal of …, 2020 - Am Soc Microbiol
Porcine torovirus was first detected and characterized in feces samples from piglets in the
… to be widespread in the global pig population and swine herds have a high seroprevalence, …

Development and use of a reverse transcription insulated isothermal PCR assay for detection and characterization of bovine torovirus in yaks

L Zhao, G Shao, C Tang, H Yue - Archives of Virology, 2021 - Springer
… Bovine torovirus (BToV) is an important diarrhea-causing pathogen affecting … ) assay was
developed that targets the BToV M gene with high specificity and reproducibility. The assay has …

Current state of molecular and serological methods for detection of porcine epidemic diarrhea virus

M Olech - Pathogens, 2022 - mdpi.com
amplification assays. Additionally, several serological methods have been developed and
are widely used for the detection of antibodies against PEDV. Some of them, such as the …

Characterization and identification of a novel torovirus associated with recombinant bovine torovirus from Tibetan antelope in Qinghai-Tibet Plateau of China

X Dai, S Lu, G Shang, W Zhu, J Yang, L Liu… - Frontiers in …, 2021 - frontiersin.org
… and comprise three species, equine torovirus (EToV), bovine torovirus (BToV), and porcine
torovirus (PToV). In this study, a novel torovirus (antelope torovirus, AToV) was discovered …

Development of duplex LAMP technique for detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 2 (PCV 2)

S Areekit, P Tangjitrungrot… - Current Issues in …, 2022 - mdpi.com
Porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 2 (PCV2) are both …
on the swine industry. In this study, a duplex loop-mediated isothermal amplification (duplex …

Progress of research on coronaviruses and toroviruses in large domestic animals using reverse genetics systems

M Ujike, T Suzuki - The Veterinary Journal, 2024 - Elsevier
… animals such as pigs and cattle. In the last decade, however, studies of porcine CoVs and
bovine … made in the last decade in RGS-based research on porcine CoVs and bovine ToVs. …

First Identification and Pathogenicity Evaluation of an EV-G17 Strain Carrying a Torovirus Papain-like Cysteine Protease (PLCP) Gene in China

ZH Li, ZR Li, P Zhu, ZX Zhang, JL Song - Viruses, 2023 - mdpi.com
… cysteine protease (PLCP) gene of porcine torovirus have been isolated or detected, while
… In this study, an EV-G17-PLCP strain, ‘EV-G/YN23/2022’, was isolated from the feces of pigs