CRISPR-mediated gene-editing technology has arguably driven an unprecedented
revolution in biological sciences for its role in elucidating gene functions. A multitude of …

ABSTRACT CRISPR-mediated gene-editing technology has arguably driven an
unprecedented revolution in biological sciences for its role in elucidating gene functions. A …

Targeted genome editing is now possible in nearly any organism and is widely
acknowledged as a biotech game-changer. Among available gene editing techniques, the …

Clustered regularly interspaced short palindromic repeats (CRISPR) target sequence
selection is a key step for achieving successful targeted gene editing. Possible off-target …

The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated
protein 9 (CRISPR-Cas9) system has emerged as a versatile molecular tool for genome …

A large number of computational tools have been documented in recent years for
identification of target-specific valid single-guide (sg) RNAs (18–20 nucleotide long …

This chapter discusses the general rules for selecting target sites for genome editing using
the Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas technology …

The CRISPR/Cas endonuclease system, generally used as a defense mechanism in
bacteria and archaea against invading phages, has emerged as a potential editing tool in …

The CRISPR/Cas9 system has been developed as a powerful technology for both targeted
genome editing and gene regulation. However, the design of efficient single-guide RNAs …

CRISPR/Cas-based genome editing in any biological application requires the evaluation of
suitable genomic target sites to design efficient reagents. Considerations for the design of …