Metabolic Engineering of Clostridium cellulovorans to Improve Butanol Production by Consolidated Bioprocessing

Z Wen, R Ledesma-Amaro, M Lu, M Jin… - ACS synthetic …, 2020 - ACS Publications
Clostridium cellulovorans DSM 743B can produce butyrate when grown on lignocellulose,
but it can hardly synthesize butanol. In a previous study, C. cellulovorans was successfully …

Improved n-Butanol Production from Clostridium cellulovorans by Integrated Metabolic and Evolutionary Engineering

Z Wen, R Ledesma-Amaro, J Lin… - Applied and …, 2019 - Am Soc Microbiol
Clostridium cellulovorans DSM 743B offers potential as a chassis strain for biomass refining
by consolidated bioprocessing (CBP). However, its n-butanol production from lignocellulosic …

Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline cellulose

SM Gaida, A Liedtke, AHW Jentges, B Engels… - Microbial cell …, 2016 - Springer
Background Sustainable alternatives for the production of fuels and chemicals are needed
to reduce our dependency on fossil resources and to avoid the negative impact of their …

Engineering Clostridium cellulovorans for highly selective n‐butanol production from cellulose in consolidated bioprocessing

T Bao, W Hou, X Wu, L Lu, X Zhang… - Biotechnology and …, 2021 - Wiley Online Library
Cellulosic n‐butanol from renewable lignocellulosic biomass has gained increased interest.
Previously, we have engineered Clostridium cellulovorans, a cellulolytic acidogen, to …

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium

Z Wen, NP Minton, Y Zhang, Q Li, J Liu, Y Jiang… - Metabolic …, 2017 - Elsevier
The efficient fermentative production of solvents (acetone, n-butanol, and ethanol) from a
lignocellulosic feedstock using a single process microorganism has yet to be demonstrated …

Strategies for improved isopropanol–butanol production by a Clostridium strain from glucose and hemicellulose through consolidated bioprocessing

F Xin, T Chen, Y Jiang, W Dong, W Zhang… - Biotechnology for …, 2017 - Springer
Background High cost of traditional substrates and formation of by-products (such as
acetone and ethanol) in acetone–butanol–ethanol (ABE) fermentation hindered the large …

n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases

T Bao, J Zhao, J Li, X Liu, ST Yang - Bioresource technology, 2019 - Elsevier
With high cellulolytic and acetic/butyric acids production abilities, Clostridium cellulovorans
is promising for use to produce cellulosic n-butanol. Here, we introduced three different …

Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose

X Yang, M Xu, ST Yang - Metabolic Engineering, 2015 - Elsevier
Production of cellulosic biofuels has drawn increasing attention. However, currently no
microorganism can produce biofuels, particularly butanol, directly from cellulosic biomass …

Efficient bio-butanol production from lignocellulosic waste by elucidating the mechanisms of Clostridium acetobutylicum response to phenolic inhibitors

H Luo, P Zheng, M Bilal, F Xie, Q Zeng, C Zhu… - Science of the total …, 2020 - Elsevier
Lignocellulosic biomass is considered abundant renewable feedstock to constitute a green
and environmentally friendly approach for biofuels (bio-butanol) production as an effective …

Combined evolutionary engineering and genetic manipulation improve low pH tolerance and butanol production in a synthetic microbial Clostridium community

Z Wen, R Ledesma‐Amaro, M Lu… - Biotechnology and …, 2020 - Wiley Online Library
Synthetic microbial communities have become a focus of biotechnological research since
they can overcome several of the limitations of single‐specie cultures. A paradigmatic …