The polyadenylation of RNA is a near-universal feature of RNA metabolism in eukaryotes.
This process has been studied in the model alga Chlamydomonas reinhardtii using low …

It is well known that nearly all eukaryotic mRNAs contain a 3′ poly (A) tail. A
polyadenylation signal (AAUAAA) nearby the 3′ end of pre-mRNA is required for poly (A) …

Recent studies have uncovered new connections between the enzymes of mRNA 3′ end
processing and RNA polymerase II. These connections improve the efficiency of …

Ends of almost all eukaryotic mRNAs are generated by endonucleolytic cleavage and
addition of a poly (A) tail. In mammalian cells, the reaction depends on the sequence …

Polyadenylation of mRNA precursors by poly (A) polymerase depends on a specificity factor,
CPF, recognizing the polyadenylation signal AAUAAA. This paper describes an apparently …

An SP6 RNA containing the adenovirus 5 L3 poly (A) site is processed efficiently in a HeLa
cell nuclear extract to generate correct 3'termini. Accurate 3'processing has also been …

Polyadenylation of mRNA precursors by poly (A) polymerase depends on two specificity
factors and their recognition sequences. These are cleavage and polyadenylation specificity …

To determine whether a specific nucleotide sequence is required to direct polyadenylation of
a simian virus 40 early pre-mRNA in a soluble HeLa whole-cell lysate, we constructed a …

Poly (A)-specific ribonuclease (PARN) is a mammalian 3′-exoribonuclease that degrades
poly (A) with high specificity. To reveal mechanisms by which poly (A) is recognized by the …

Publisher Summary This chapter discusses the enzymes that are likely to be responsible for
synthesis of poly (A) sequences and has led to new methods for obtaining preparations of …