During the synthesis of mRNA in the nucleus, 3′-ends are generated by endonucleolytic
cleavage followed by polyadenylation. The machinery responsible for this simple reaction is …

Pre–messenger RNA (mRNA) 3′-end cleavage and subsequent polyadenylation strongly
regulate gene expression. In comparison with the upstream or downstream motifs, relatively …

Pre‐mRNA cleavage and polyadenylation is an essential step for 3′ end formation of
almost all protein‐coding transcripts in eukaryotes. The reaction, involving cleavage of …

Poly (A) tails of mRNAs are synthesized in the cell nucleus with a defined length,∼ 250
nucleotides in mammalian cells. The same type of length control is seen in an in vitro …

Expression of mature messenger RNA s (mRNAs) requires appropriate transcription
initiation and termination, as well as pre‐mRNA processing by capping, splicing, cleavage …

Obtaining pure mRNA preparations from prokaryotes has been difficult, if not impossible, for
want of a poly (A) tail on these messages. We have used poly (A) polymerase from yeast to …

Poly (A) tails have long been known as stable 3′ modifications of eukaryotic mRNAs,
added during nuclear pre‐mRNA processing. It is now appreciated that this modification is …

In eukaryotic cells, newly synthesized mRNAs acquire a poly (A) tail that plays several
fundamental roles in export, translation and mRNA decay. In mammals, PABPN1 controls …

Genes containing multiple pre‐mRNA cleavage and polyadenylation sites, or polyA sites,
express mRNA isoforms with variable 3′ untranslated regions (UTRs). By systematic …