[HTML][HTML] Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi

MJ Kwon, T Schütze, S Spohner, S Haefner… - Fungal biology and …, 2019 - Springer
Background Within the last years, numerous reports described successful application of the
CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi. However, still a …

[HTML][HTML] CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective

R Song, Q Zhai, L Sun, E Huang, Y Zhang… - Applied microbiology …, 2019 - Springer
Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

[HTML][HTML] Cpf1 enables fast and efficient genome editing in Aspergilli

KG Vanegas, ZD Jarczynska, T Strucko… - Fungal Biology and …, 2019 - Springer
Background CRISPR technology has revolutionized fungal genetic engineering by
increasing the speed and complexity of the experiments that can be performed. Moreover …

[HTML][HTML] CRISPR-Cas9 genome editing approaches in filamentous fungi and oomycetes

M Schuster, R Kahmann - Fungal Genetics and Biology, 2019 - Elsevier
Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

[HTML][HTML] Development of a versatile and conventional technique for gene disruption in filamentous fungi based on CRISPR-Cas9 technology

YM Zheng, FL Lin, H Gao, G Zou, JW Zhang… - Scientific reports, 2017 - nature.com
Filamentous fungi represent an invaluable source of pharmaceutically active compounds.
The development of versatile methods to genetically manipulate filamentous fungi is of great …

[HTML][HTML] Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system

R Liu, L Chen, Y Jiang, Z Zhou, G Zou - Cell Discovery, 2015 - nature.com
Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a
powerful genome-editing method that facilitates genetic alterations of genomes in a variety …

CRISPR/Cas9‐based genome engineering: A new breakthrough in the genetic manipulation of filamentous fungi

M Ullah, L Xia, S Xie, S Sun - Biotechnology and Applied …, 2020 - Wiley Online Library
Filamentous fungi have several industrial, environmental, and medical applications.
However, they are rarely utilized owing to the limited availability of full‐genome sequences …

Molecular tools for gene manipulation in filamentous fungi

S Wang, H Chen, X Tang, H Zhang, W Chen… - Applied microbiology …, 2017 - Springer
Functional genomics of filamentous fungi has gradually uncovered gene information for
constructing 'cell factories' and controlling pathogens. Available gene manipulation methods …

CRISPR/Cas9-based genome editing of the filamentous fungi: the state of the art

TQ Shi, GN Liu, RY Ji, K Shi, P Song, LJ Ren… - Applied microbiology …, 2017 - Springer
In recent years, a variety of genetic tools have been developed and applied to various
filamentous fungi, which are widely applied in agriculture and the food industry. However …

[HTML][HTML] Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277

D Abdulrachman, L Eurwilaichitr, V Champreda… - BMC …, 2021 - Springer
Background CRISPR-Cas genome editing technologies have revolutionized
biotechnological research particularly in functional genomics and synthetic biology. As an …