Bioluminescence has long been used to image biological processes in vivo. This technology
features luciferase enzymes and luciferin small molecules that produce visible light …

Highlights•Advances in bioluminescent tool production that mirror trends in fluorescent
probe development.•New bioluminescent platforms based on naturally occurring luciferases …

Bioluminescence with luciferase–luciferin pairs is an attractive method for surveying cells in
live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase …

Bioluminescence (BL) relies on the enzymatic reaction between luciferase, a substrate
conventionally named luciferin, and various cofactors. BL imaging has become a widely …

Conspectus Bioluminescence is widely used for real-time imaging in living organisms. This
technology features a light-emitting reaction between enzymes (luciferases) and small …

Bioluminescence is the fascinating natural phenomenon by which living creatures produce
light. Bioluminescence occurs when the oxidation of a small-molecule luciferin is catalysed …

Bioluminescence imaging (BLI) exploits the light-emitting properties of luciferase enzymes
for monitoring cells and biomolecular processes in living subjects. Luciferases can be …

Luciferase enzymes catalyze the emission of light from a substrate—a phenomenon known
as bioluminescence—and have been employed as reporters of many biological functions …

Although fluorescent reporters and biosensors have become indispensable tools in
biological and biomedical fields, fluorescence measurements require external excitation …

Bioluminescence imaging with luciferase–luciferin pairs is widely used in biomedical
research. Several luciferases have been identified in nature, and many have been adapted …