Morphometric analysis of sperm used for IVP by three different separation methods with spatial light interference microscopy

M Rubessa, ME Kandel, S Schreiber… - Systems Biology in …, 2020 - Taylor & Francis
The goal of this study was to characterize sperm populations resulting from three different
methods of sperm selection used for bovine in vitro fertilization. We compared sperm …

High-throughput sperm assay using label-free microscopy: morphometric comparison between different sperm structures of boar and stallion spermatozoa

M Rubessa, JM Feugang, ME Kandel… - Animal reproduction …, 2020 - Elsevier
The capacity for microscopic evaluation of sperm is useful for assisted reproductive
technologies (ART), because this can allow for specific selection of sperm cells for in vitro …

252 comparison of two sperm separation products for use in bovine IVF

J Pryor, S Romo, DD Varner, K Hinrichs… - Reproduction, Fertility …, 2004 - CSIRO Publishing
In commercial bovine in vitro fertilization (IVF) companies, there is a continuous need to
improve results. Efforts to maximize in vitro embryo production have included modifications …

[HTML][HTML] Interferometric phase microscopy for label-free morphological evaluation of sperm cells

M Haifler, P Girshovitz, G Band, G Dardikman… - Fertility and sterility, 2015 - Elsevier
Objective To compare label-free interferometric phase microscopy (IPM) to label-free and
label-based bright-field microscopy (BFM) in evaluating sperm cell morphology. This …

Bovine spermatozoal head size variation and evaluation of a separation technique based on this size

JE Chandler, MP Wilson, AM Canal… - Theriogenology, 1999 - Elsevier
The objectives of this study were to determine the variation of head areas of normal
spermatozoa attributable to breed, individual bull and ejaculate and to verify separation of X …

Use of microfluidics to sort stallion sperm for intracytoplasmic sperm injection

RA Gonzalez-Castro, EM Carnevale - Animal reproduction science, 2019 - Elsevier
We determined if microfluidic sorting (MF) of frozen-thawed stallion sperm improves sperm
population characteristics and results in embryo development after intracytoplasmic sperm …

In vitro survival kinetics of microfluidic‐sorted bovine spermatozoa

K Ogata, MPB Nagata, H Nishizono, T Yamanouchi… - …, 2021 - Wiley Online Library
Background The isolation and characterization of sperm subpopulations that can achieve
fertilization is a major challenge of assisted reproduction methods. We focused on the …

Comparative study of sperm washing and selection methods after cryopreservation and its influence on sperm subpopulational structure in a bovine model

M García-Herreros, CLV Leal - Systems biology in reproductive …, 2014 - Taylor & Francis
The effect of different sperm washing-selection methods on sperm morphometric
characteristics as a study to detect differences in the subpopulational structure has been …

Sperm selection techniques in cattle: Microfilter device versus conventional methods

J Vega‐Hidalgo, M Rodriguez, D Dipaz‐Berrocal… - Andrologia, 2022 - Wiley Online Library
Microfluidics and microfilter devices have been developed to mimic the characteristics of the
female reproductive tract, minimizing the risk of sperm damage. This study aimed to …

Progressive bovine sperm separation using parallelized microchamber-based microfluidics

M Yaghoobi, M Azizi, A Mokhtare, A Abbaspourrad - Lab on a Chip, 2021 - pubs.rsc.org
Motility is one of the most important factors in sperm migration toward an egg. Therefore,
sperm separation based on motility might enhance sperm selection for infertility treatments …