Standardisation of genetic parts has become a topic of increasing interest over the last
decades. The promise of simplifying molecular cloning procedures, while at the same time …

Saccharomyces cerevisiae is an established industrial host for production of recombinant
proteins, fuels and chemicals. To enable stable integration of multiple marker‐free …

The yeast S. cerevisiae serves as a model organism for many decades. Numerous
molecular tools have been developed throughout the years to engineer its genome …

CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression
plasmids for multiplexed gene knockouts and point mutations. Here we show that co …

Synthetic biology toolkits are one of the core foundations on which the field has been built,
facilitating and accelerating efforts to reprogram cells and organisms for diverse …

Over the past decade, a new strategy was developed to bypass the difficulties to genetically
engineer some microbial species by transferring (or “cloning”) their genome into another …

Saccharomyces cerevisiae continues to serve as a powerful model system for both basic
biological research and industrial application. The development of genome-wide collections …

Demands on the industrial and academic yeast strain engineer have increased significantly
in the era of synthetic biology. Installing complex biosynthetic pathways and combining point …

The preparation of genetic libraries is an essential step to evolve microorganisms and study
genotype-phenotype relationships by high-throughput screening/selection. As the large …

Thanks to its sophistication, the CRISPR/Cas system has been a widely used yeast genome
editing method. However, CRISPR methods generally rely on preassembled DNAs and …