[HTML][HTML] Comparison and sensitivity evaluation of three different commercial real-time quantitative PCR kits for SARS-CoV-2 detection

A Banko, G Petrovic, D Miljanovic, A Loncar… - Viruses, 2021 - mdpi.com
Real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most sensitive
and specific assay and, therefore, is the “gold standard” diagnostic method for the diagnosis …

[HTML][HTML] Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR

M Park, J Won, BY Choi, CJ Lee - Experimental & molecular medicine, 2020 - nature.com
SARS-CoV-2 is very contagious and has rapidly spread globally. Due to various
symptomatic and asymptomatic cases and the possibility of asymptomatic transmission …

Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples

M Pereira-Gómez, Á Fajardo, N Echeverría… - Journal of virological …, 2021 - Elsevier
The pandemic caused by SARS-CoV-2 has triggered an extraordinary collapse of
healthcare systems and hundred thousand of deaths worldwide. Following the declaration of …

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?

A Anantharajah, R Helaers, JP Defour, N Olive… - Journal of virological …, 2021 - Elsevier
Abstract Objectives The SARS-CoV-2 pandemic has created an unprecedented need for
rapid large-scale diagnostic testing to prompt clinical and public health interventions …

Comparison of the diagnostic efficacy between two PCR test kits for SARS‐CoV‐2 nucleic acid detection

Y Lu, L Li, S Ren, X Liu, L Zhang… - Journal of clinical …, 2020 - Wiley Online Library
Background To compare the diagnostic efficacy between two different real‐time reverse
transcription polymerase chain reaction (RT‐PCR) test kits for severe acute respiratory …

[HTML][HTML] Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories

JJ LeBlanc, JB Gubbay, Y Li, R Needle… - Journal of Clinical …, 2020 - Elsevier
With emergence of pandemic COVID-19, rapid and accurate diagnostic testing is essential.
This study compared laboratory-developed tests (LDTs) used for the detection of SARS-CoV …

[HTML][HTML] Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA

WG Dundon, TBK Settypalli, K Spiegel… - Journal of virological …, 2021 - Elsevier
Transmission mitigation of SARS-CoV-2 requires the availability of accurate and sensitive
detection methods. There are several commercial ad hoc molecular diagnostic kits currently …

Rapid and sensitive detection of SARS-CoV-2 RNA using the Simplexa™ COVID-19 direct assay

L Bordi, A Piralla, E Lalle, F Giardina, F Colavita… - Journal of Clinical …, 2020 - Elsevier
Background So far, one of the major drawbacks of the available molecular assays for the
diagnosis of severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) is the need …

[HTML][HTML] A novel multiplex qRT-PCR assay to detect SARS-CoV-2 infection: high sensitivity and increased testing capacity

S Petrillo, G Carrà, P Bottino, E Zanotto, MC De Santis… - Microorganisms, 2020 - mdpi.com
Rapid and sensitive screening of severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2) is essential to limit the spread of the global pandemic we are facing. Quantitative real …

[HTML][HTML] Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2

H Tombuloglu, H Sabit, E Al-Suhaimi, R Al Jindan… - Plos one, 2021 - journals.plos.org
The outbreak of the new human coronavirus SARS-CoV-2 (also known as 2019-nCoV)
continues to increase globally. The real-time reverse transcription polymerase chain …