The potential benefits of gene therapy for neurological diseases such as Parkinson's,
Amyotrophic Lateral Sclerosis (ALS), Epilepsy, and Alzheimer's are enormous. Even a delay …

The technology of expressing foreign genes in mammalian cells has become increasingly
important to study a number of biological questions and as a primary method for production …

Conventional cancer and transformed cell lines are widely used in cancer biology and other
fields within biology. These cells usually have abnormalities from the original tumor itself, but …

The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) can infect
a variety of mammalian cells, as well as insect cells, facilitating its use as a viral vector for …

This chapter describes methods for growing, purifying, counting, and characterizing viruses.
It also provides general principles of diagnostic virology. As obligate intracellular parasites …

The development of viral vectors for gene therapy has had an interesting and somewhat
tumultuous history but remains the most important avenue tor treatment of a large number of …

Although viruses are simple biological systems, they are capable of evolving highly efficient
techniques for infecting cells, expressing their genomes, and generating new copies of …

Virus replication requires specific interactions with host cells. The replication cycle begins
with attachment of viral proteins to host cell receptors. The presence or absence of receptors …

Gene therapy is now being applied to the treatment of a wide variety of acquired and
inherited diseases. One of the rate-limiting steps for successful gene therapy is the efficiency …

Various viral vectors have been explored as vehicles to deliver therapeutic genes for human
gene therapy. Murine leukemia virus (MLV), adenovirus, and adeno associated virus (AAV) …