Secretory production of spider silk proteins in metabolically engineered Corynebacterium glutamicum for spinning into tough fibers

Q Jin, F Pan, CF Hu, SY Lee, XX Xia, ZG Qian - Metabolic engineering, 2022 - Elsevier
Spider dragline silk is a remarkable fiber made of unique proteins—spidroins—secreted and
stored as a concentrated aqueous dope in the major ampullate gland of spiders. This feat …

Host systems for the production of recombinant spider silk

DR Whittall, KV Baker, R Breitling, E Takano - Trends in Biotechnology, 2021 - cell.com
Spider silk is renowned for its impressive mechanical properties. It is one of the strongest
known biomaterials, possessing mechanical properties that outmatch both steel and Kevlar …

[HTML][HTML] High-yield production of a super-soluble miniature spidroin for biomimetic high-performance materials

B Schmuck, G Greco, A Barth, NM Pugno, J Johansson… - Materials Today, 2021 - Elsevier
The mechanical properties of artificial spider silks are approaching a stage where
commercial applications become realistic. However, the yields of recombinant silk proteins …

Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber

XX Xia, ZG Qian, CS Ki, YH Park… - Proceedings of the …, 2010 - National Acad Sciences
Spider dragline silk is a remarkably strong fiber that makes it attractive for numerous
applications. Much has thus been done to make similar fibers by biomimic spinning of …

Unconventional spidroin assemblies in aqueous dope for spinning into tough synthetic fibers

CF Hu, ZG Qian, Q Peng, Y Zhang… - … Biomaterials Science & …, 2021 - ACS Publications
Spider dragline silk is a remarkable fiber made by spiders from an aqueous solution of
spidroins, and this feat is largely attributed to the tripartite domain architecture of the silk …

Hyper-production of large proteins of spider dragline silk MaSp2 by Escherichia coli via synthetic biology approach

YX Yang, ZG Qian, JJ Zhong, XX Xia - Process Biochemistry, 2016 - Elsevier
Spider dragline silk exhibits excellent mechanical properties that make it a promising protein
polymer for industrial and biomedical applications. Since farming spiders is not feasible due …

Engineered spider silk proteins for biomimetic spinning of fibers with toughness equal to dragline silks

T Arndt, G Greco, B Schmuck, J Bunz… - Advanced Functional …, 2022 - Wiley Online Library
Spider silk is the toughest fiber found in nature, and bulk production of artificial spider silk
that matches its mechanical properties remains elusive. Development of miniature spider …

Engineered spidroin-derived high-performance fibers for diverse applications

D Qin, J Li, H Li, H Zhang, K Liu - Nano Research, 2024 - Springer
Spider silks are well known for their exceptional mechanical properties that are tougher than
Kevlar and steel. However, the restricted production amounts from their native sources limit …

Toward spinning artificial spider silk

A Rising, J Johansson - Nature chemical biology, 2015 - nature.com
Spider silk is strong and extensible but still biodegradable and well tolerated when
implanted, making it the ultimate biomaterial. Shortcomings that arise in replicating spider …

Spider silk proteins: recent advances in recombinant production, structure–function relationships and biomedical applications

A Rising, M Widhe, J Johansson… - Cellular and Molecular …, 2011 - Springer
Spider dragline silk is an outstanding material made up of unique proteins—spidroins.
Analysis of the amino acid sequences of full-length spidroins reveals a tripartite composition …