With the demand for green, safe, and continuous biocatalysis, bioscaffolds, compared with
synthetic scaffolds, have become a desirable candidate for constructing enzyme …

The utilization of scaffolds for enzyme immobilization involves advanced bionanotechnology
applications in biorefinery fields, which can be achieved by optimizing the function of various …

Enzymes tend to malfunction when they work out of their natural cellular environments.
Engineering a favorable microenvironment around enzymes has emerged as an effective …

In a biological system, the spatiotemporal arrangement of enzymes in a dense cellular
milieu, subcellular compartments, membrane-associated enzyme complexes on cell …

Background When using the microbial cell factories for green manufacturing, several
important issues need to be addressed such as how to maintain the stability of biocatalysts …

Co-immobilization of multi-enzymes has emerged as a promising concept to design and
signify bio-catalysis engineering. Undoubtedly, the existence and importance of basic …

Single enzyme systems or engineered microbial hosts have been used for decades but the
notion of assembling multiple enzymes into cell-free synthetic pathways is a relatively new …

Immobilization depicts a propitious route to optimize the catalytic performances, efficient
recovery, minimizing autocatalysis, and also augment the stabilities of enzymes, particularly …

Nature relies on complexes of colocated enzymes to efficiently perform multiple catalytic
steps. Such enzyme colocalisation promotes substrate channelling, enhances the activity of …

In nature, metabolic pathways are often organized into complex structures such as
multienzyme complexes, enzyme molecular scaffolds, or reaction microcompartments …