Ca2+-triggered coelenterazine-binding protein (CBP) is a natural form of the luciferase
substrate involved in the Renilla bioluminescence reaction. It is a stable complex of …

The Renilla bioluminescent system in vivo is comprised of three proteins—the luciferase,
green-fluorescent protein, and coelenterazine-binding protein (CBP), previously called …

It has been shown that the coelenterazine analog, coelenterazine-v, is an efficient substrate
for a reaction catalyzed by Renilla luciferase. The resulting bioluminescence emission …

The recombinant Ca 2+-triggered coelenterazine-binding protein (CBP) from Renilla
muelleri was investigated as a biospecifically labeled molecule for in vitro assay …

The functioning of bioluminescent systems in most of the known marine organisms is based
on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by …

Bioluminescence is a widespread natural phenomenon. Luminous organisms are found
among bacteria, fungi, protozoa, coelenterates, worms, molluscs, insects, and fish. Studies …

Bioluminescence-based techniques, such as bioluminescence imaging, BRET and dual-
luciferase reporter assay systems, have been widely used to examine a myriad of biological …

Recent advances in bioluminescence technology rely on the discovery of luciferin-luciferase
pairs having excellent luminescence activity. Here, we focus on coelenterazine (CTZ) and its …

The cold-induced expression system in Escherichia coli is useful and we have applied this
system to prepare the coelenterazine-utilizing luciferases including Renilla luciferase …

Blue fluorescent protein from the calcium-binding photoprotein aequorin (BFP-aq) is a
dissociable complex of Ca2+-bound apoaequorin and coelenteramide, and is identified as a …